EMAGE mouse gene expression spatial database

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EMAGE:7693

Ints9 integrator complex subunit 9 ( MGI:1098533)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005301. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005301_01	euxassay_005301_02	euxassay_005301_03	euxassay_005301_04

euxassay_005301_05	euxassay_005301_06	euxassay_005301_07	euxassay_005301_08	euxassay_005301_09

euxassay_005301_10	euxassay_005301_11	euxassay_005301_12	euxassay_005301_13	euxassay_005301_14

euxassay_005301_15	euxassay_005301_16	euxassay_005301_17	euxassay_005301_18	euxassay_005301_19

euxassay_005301_20	euxassay_005301_21	euxassay_005301_22	euxassay_005301_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7693_wholemount_strong.wlz

7693_wholemount_moderate.wlz

7693_wholemount_weak.wlz

7693_wholemount_possible.wlz

7693_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7693_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8960
Entity Detected:	Ints9, integrator complex subunit 9 ( MGI:1098533)
Sequence:	sense strand is shown >T8960 CCAAAAGTCCGATATGAAACTGTATTGCCTGTCAGGGCACCCAACTTTACCATGCAACGTACTCAAATTC AAATCCACTACTATTATGTTGGACTGTGGACTGGACATGACTTCCACCCTCAATTTCCTCCCTTTGCCTC TTGTTCAAAGCCCCAGGCTATCTAATCTTCCCGGATGGTCTCTAAAAGACGGAAATGCTTTTTTGGACAA GGAGCTAAAAGAGTGCTCCGGTCATGTATTTGTGGATTCTGTGCCTGAATTCTGCTTACCAGAGACCGAG CTAATAGACCTGTCTACAGTCGATGTGATCCTCATCTCTAACTACCACTGCATGATGGCGCTGCCCTACA TCACCGAGCACACAGGATTCACAGGCACAGTGTATGCCACAGAACCCACCATGCAGATCGGCAGGCTTCT CATGGAAGAGCTGGTGAATTTCATCGAAAGAGTGCCAAAGGCCCAGTCTGCCTCCTTGTGGAAGAATAAG GATATACAGCGGCTGCTGCCTTCTCCCCTCAAGGATGCAGTGGAAGTGTCAACCTGGAGAAGATGCTACA CGATGCAGGAGGTGAACTCGGCCCTCAGTAAAATCCAGCTGGTGGGATATTCCCAGAAAATCGAGCTGTT TGGTGCTGTCCAGGTGACCCCTCTGAGCTCTGGCTATGCCCTTGGAAGCTCCAACTGGATTATCCAGTCA CATTATGAGAAAGTATCTTACGTCTCTGGATCCTCCTTGCTTACCACACACCCTCAGCCCATGGACCAAG CTTCTCTCAAAAACAGTGACGTTCTGATTCTCACAGGGCTGACACAGATCCCTACTGCAAACCCGGACGG CATGGTGGGCGAGTTCTGCAGCAACCTCGCTCTGACAGTTCGGAATGGAGGAAACGTGTTGGTCCCTTGC TATCCTTCAGGCGTGATCTATGACCTCCTGGAGTGCTTATATCAATACATTGACTCCGCTGGCCTCTCCA ACATCCCTTTCTACTTCATCTCCCCCGTTGCCAACAGTTCACTCGAGTTTTCCCAGATTTTTGCTGAGTG GCTTTGTCACAACAAACAGAGTAAAGTTTACCTCCCAGAACCACCTTTCCCACATGCAGAGCTCATTCAG ACGAATAAGCTGAAGCACTACCGCAGCATCCACGGTGACTTCAGCAATGACTTCAGACAGCCGTGTGTGC TGTTCACCGGACATCCTTCCCTCCGCTTTGGGGACGTCGTCCACTTCATGGAGCTCTGGGGGAAGTCCAG CCTCAACACCATCATATTCACAGAACCCGATTTCTCCTACCTGGAGGCCTTGGCTCCTTACCAGCCGCTG GCCATGAAGTGCATCTACTGCCCAATTGACACTCGACTGAATTTCATCCAGGTGTCAAAGCTGCTTAAAG AAGTACAGCCCCTGCACGTGGTCTGTCCTGAGCAGTACACCCAGCCACCGCCAGCCCAGGCCCACAGGAT GGACCTCATGATCGACTGCCAGCCACCAGCCATGTCCTATCGACGGGCTGAGGTCCTTGCCCTGCCCTTC AAACGACGCTATGAGAAAATTGAAATCATGCCAGAGCTGGCAGACTCCCTGGTGCCCATGGAAATCAAGC CTGGCATCTCCTTGGCGACTGTCTCAGCTGTGCTGCATACGAAGGATAACAAACATGTGCTTCAGCCCCC TCCCAAGCCCACCCAGCCCACCAGCAGTAAGAAGAGGAAGCGGGTGAACGAGGACATTCCTGACTGCAAA GTACTGAAGCCTCTGCTGAGTGGCTCCATCCCCGTAGAGCAGTTTGTGCAGACCCTGGAGAAGCATGGCT TCAGTGACATTAAGGTGGAAGACACAGCCAAGGGACATATTGTCCTGCTGCAGGAAGCCGAGACACTCAT CCAGATTGAAGAAGACTCCACTCACATCATCTGTGACAATGACGAGACACTGAGGGTCCGACTGCGGGAC CTTGTCCTCAGGTTCCTGCAGAAGTTCTGAGTGGCATCCGTGACATCCTAGGCCCTTCACCCAGAGACAG TCTGGTGGTGTCCTATGACTTCCGGAGAGGAGAGGTGTCTCCAGCTAGGATCTGCCAGTGTGACTTCCTC GTACTGCTGACCCCAAGAACGTTTAGAGGCCTGTTCTTTTCAGTTCTGGGCTCCTGAGCAGCTATGGGTG ACAACATGACAGACATCCTTTCCTAAGGGAAATGAAAAAAAAAAAATGCCTGGCCTGTGTTTTTGGGGCT CACAGGGGGTGACTCTCGGACTTACCCTCCTACAGTGCTACACCGTATAAAGGTCAGCCCCCAAATTAGA AGGCAAACCTATCTCGCTGTTCTGTGGCCCGTTTTGCTAAAAATAAACATTATTTTTATAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6808816 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6808816 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7692 same embryo
	EMAGE:7691 same embryo
	EMAGE:7688 same embryo
	EMAGE:7690 same embryo
	EMAGE:7689 same embryo
	EurExpress:euxassay_005301 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source