EMAGE mouse gene expression spatial database

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EMAGE:7701

Zfp952 zinc finger protein 952 ( MGI:2441928)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007460. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007460_01	euxassay_007460_02	euxassay_007460_03	euxassay_007460_04

euxassay_007460_05	euxassay_007460_06	euxassay_007460_07	euxassay_007460_08	euxassay_007460_09

euxassay_007460_10	euxassay_007460_11	euxassay_007460_12	euxassay_007460_13	euxassay_007460_14

euxassay_007460_15	euxassay_007460_16	euxassay_007460_17	euxassay_007460_18	euxassay_007460_19

euxassay_007460_20	euxassay_007460_21	euxassay_007460_22	euxassay_007460_23	euxassay_007460_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7701_wholemount_strong.wlz

7701_wholemount_moderate.wlz

7701_wholemount_weak.wlz

7701_wholemount_possible.wlz

7701_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7701_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8967
Entity Detected:	Zfp952, zinc finger protein 952 ( MGI:2441928)
Sequence:	sense strand is shown >T8967 ACCATCTTGAAGCCGGTGCAGCTGCGGGAAGAGTCGTGTCAACTCTTCTTCGGTTACTGTCACTTCTTCT AATTTCAGGCTCCTTGGAGAGGACACAGTATGCATCAAAAGCAGAGAATGGAGCCTGTGACCTTTGAGGA TGTGGCTGTGAACTTCAGCCTAGGAGAGTGGGCTCTGCTGGATTCCAGTCAAAAGAAGCTCTACAGAGAT GTGATGACGGAGACCTTTATGAATCTGATCTCCATAGGGAAAACAGAGGAAAAAGAAAATATTGAAGACA ACTACCAAAATCTCAGGAAAAATGTGAGGACCTGGGTTGAATGTTCAGTTGGCTCATTTATATTTACAAA TTCTCACAGAAGCAAAATCAGTCGTGCCGTTCGTCCTCCCTCCTCCAGGTTCTTTGTCTATGGCCCAGAA CTCTGGTGGCTGAGAGGAGTTGTGACTACGGAGATGTCAGTCAGTGTGGAGAAACCCGGCAACGGATTCC AGAGTGTATCATTCGTAGAGACACGCCTCCTGAAATATCAGTGTGTAAAAACAGTGTGCTTGCTAGAGAC ATCCTTGTTCATTCCCTCTCAGAAGTGCACCTCAGATGTGAAACTGCAGAGAAACCCTGTGGGTCTCAGG AATATGTGAAGTGTTGCAAAGAGTCTACTTCTTCTGAGTCCTTTCAGACAAAGCAAAGTCCTCCTCGAGA GACACCCTATCAAAATAAGCAATCTAATGAAGCCTATAGGAGTCTTAATTCTGACCGGTATTGTGAGCAA ATTCACACTGGAGATAAACTCCGTGAATGGAAGCAAGTTGAGAAAGCCTTCTGGAGGTACAGTTACGGTC AAATATATGAGAGAATCACCATTGGAGAGGAACCATTTGTGTGTAAGCAGTATGGTGAAGCCTTGGTTAA TTCTAGTCATCTTATTAAACATGAAGTAATTAGCCCTGAAGAAAAATGTTACACATGTAAGCAGTGTGGG AAGGCATTCAGATATTCCTCATCCCTTCAGAACCATGAAAGAATCCATAGCGGGGAGAGGCCATATGTAT GTAAGCAATGTGGAAAAACCTTCATTCGTTCCTATGACCTTCTTATCCATGAAAGAATTCACAGTGGAGA AAAACCTTACACGTGTGAGCATTGTGGAAAATCTTTCACCCATTATAGCGGCTGGTACAGTCATGAAAGG ATTCACACTAGAGAGAAACCCTATGTGTGCACACAGTGTGGGAAAGCCTTTTCATGCTCTACATCGTTCC GGAGGCATGAAAGAATTCACACTGGAGAGAAACCTTACTCCTGTAAGCACTGTGGGAAAGCCTTCACCCA TTCCAGTGCTCGTTACATTCATGAGCGAATTCACACCGGAGAGAAACCCTACGTTTGTAAGCACTGTGGA AAAGCATTCCTCCGTGCCAGTCATCTCAACAACCATGAGAGAATTCACACTGGAGAGAAACCCTATATTT GTAAGCATTGTGGGAAAGCCTTCATCCAGCGCGATGCTTGTTATAATCACGAAAGAATTCACACTGGAGA GAAACCATATGTATGTAAGGAATGTGGAAAAGCATTTAGGATTTCCACATCCCTTCGTGACCATGAGAGA ATTCACACTGGAGAAAAACCTTACATATGTAATTATTGTGGGAAAGCATTTAGGGTCTCCACATGCCTTC ACAAACATGAACGAGCTCATGCTGAAAAGAAACCCAGTGGGTAGAACTGAAGCCCATATAGATCTTTGAT CAACTGTCAGGAAAAGTGTATGCAGGGTCAGTCAGATGGCTCAGGGATAAAAGCACTTGCCCTAAGCTTG GTAGCCATAGTTGGAACCTCAGAACCCATGTGAAGAGAGAGAACTGAATTTCCAAGGTTTGCCTCTGACT TGTACATATGTGCTGTCATTTGCATGCATACCCTCACATAGATGTATAAAGAAATAGGATAATTTAAAAT ACCTTTTTAAATTTTGTTTTGTTTTGAGACAGGGTTTCACTGTGCAGCCCCTTGGTTGTCCTGCAACTCA CTCTGCAGACCAGGCTGGCCTTAAACTCAGAGGTCCCCCTGCCTCTGCCTTCCGAGTGTTGAGATTAAAA GTGTGCACGTCCCTGCTGCCTAGCTTTAAGATCATTTTAATGTTGCTTTTGATTTGTAGTTATTTGTAGT TATGTGTGGGTATGTGCATGTGAGGGCTGGAGCACACAGCAGTCAGAGGCATCACATTCCCCAGGAGCTG GAGTTACAGGAGCCGGTGAGGTGTCCCATGTGACGGCTGGAAATCCAGCTTGGGTCCTCCGGAGAGCAGT ATATTCTGAGCCATCTTTCTAGTCTCATAGGTGTAATTAAAGACAAATTGGGAAAGAAATAGCTGGAATA GACCTTAAGATGTGTAAATTTGGACACATATGAAATGTGATGTTGGATAAAAACGAGTTCCATCAAATGT GCTAAGGCACTTTTTTTTTGGAAATTATATTTAATATAAATTATGAACACAGTTCATGAACATTACATAG GTGTGTGTGCATGGTGTCCACATGCCAGTGTGCATGTGGAATTGGACAGCTTTCAGAGGTCTGGTCTGTT CTTTTCTCTCTGTAGGCCCTAAGAAGGAACTCAGGCTGCTTTTGAAACAGGCGCCTTACCCTGCTGGGCA GTCTTGCTGTCCCTGCCTCATGCCATTTACTCATTGGAGAAGGAGATGCTAGAAAATTGCAAAATTGAAT ACAGTGGATTTGCACACACCTGGGAGGGTTAAGAACTCATTCCAACCAATAAAAAAAAATTCAGCATTAA AAATAATGTAAGTTGTATGTTGTCTCAAATAAGGTGAAAGGGAATTCTCTCCTGTGTGTTGTAAAAGCAA GAAAACTGAGCACTCCAAATAAATGCTTCCCTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6810096 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6810096 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7704 same embryo
	EMAGE:7703 same embryo
	EMAGE:7702 same embryo
	EMAGE:7705 same embryo
	EMAGE:7700 same embryo
	EurExpress:euxassay_007460 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source