EMAGE mouse gene expression spatial database

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EMAGE:7719

Taf1c TATA box binding protein (Tbp)-associated factor, RNA polymerase I, C ( MGI:109576)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007477. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007477_01	euxassay_007477_02	euxassay_007477_03	euxassay_007477_04

euxassay_007477_05	euxassay_007477_06	euxassay_007477_07	euxassay_007477_08	euxassay_007477_09

euxassay_007477_10	euxassay_007477_11	euxassay_007477_12	euxassay_007477_13	euxassay_007477_14

euxassay_007477_15	euxassay_007477_16	euxassay_007477_17	euxassay_007477_18	euxassay_007477_19

euxassay_007477_20	euxassay_007477_21	euxassay_007477_22	euxassay_007477_23	euxassay_007477_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7719_wholemount_strong.wlz

7719_wholemount_moderate.wlz

7719_wholemount_weak.wlz

7719_wholemount_possible.wlz

7719_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7719_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8994
Entity Detected:	Taf1c, TATA box binding protein (Tbp)-associated factor, RNA polymerase I, C ( MGI:109576)
Sequence:	sense strand is shown >T8994 GCGGCGTTGAGGTTCGGCACTCTGGCTCCTTTGCGGCCCCGGGGTCAGGATGGACTTCCCTGGCACCCTG CGCCCTTCATTGTTTAAGGCCGGTCCACTTGGCATGACTGATGGCCCTGACCTGTCCTTCATGTGCAGCT GGAGAGATGCACTGACCCTGCCAGGGTCCCAGCCACAGAACTGCAAGGATCCGACCTTGTCCTTTGCCAA GAACCTACTGTGGGAGCCATCGACGCCAGGACCCCTCCCCCTCCTGATGCCTCCTGATCCTGATCCCTGG GACCCTGGTGTGACAGCTCAGGACTTCCTTTTCCGGGGAGGTCACTGCTACCAGTACCAGTCTCAGGCTG TGCTGGATGTCACTGAGCAGCTCAGCCGCTTCCTGTGGGACCATGGGGACATAGCCTTCGCACCCCTGGG GAGACTCATGCTGGAGAACTTCAGATTGGAGGGGAACCGGGGCTACTCGAAAAAGATGACTATAGTCAGC GCGAAGAAGCTGCTTCAGGACCTCGGCGGACACCAACCCTGGGGGTGTCCCTGGGCTTCCCTCAGCCGCC GGCTGCGTCGCTTCTCCATCGTTGGGGGTCCTGTCCTGAGCAGGTCAGTGTCACTACTCATGGGGAAGCT ACTGCATGAAGAGCTGGCCATGCGCTGGGAGCAGCTGCTGATGGATGAGGCCTTCACCGGGGGCGCGTTG GCCTGGCTGCCCGGAAGGACAGCGCGGGCCGGACAGCTGGTCTACCCTAGTGGAGGTGCCTTGGACAAGC TGTATTTCCAGGAGGTCAGTGTGACTTCAGGTGGCAACCCCCGGATCCTTGAGAACCCTGGGCACGTCCA GCTGCGGGGACCTGTACGGCAGGTGGTGACCAGCACCGTCCAGGGAGAAACGCTTCTGGCCGTCCGCTCT GACTATCACTGTGCCACGTGGAAGATTGATAAGCAGGGGCCACCAGCACTCCTGCAGGTGATGCAGGTGG AGAAAGGGGCCACTGGTATTAGCCTCAGCCCTCACCTGTCAGGAGAGCTGGCCATCTGCAGCCGTTCCGG AGCTGTTTGTCTGTGGACCCCACAAGCTGGGCTGCAGACGATCTACAAGGACACCGAGACGCTTGCCTTC CGTGATCCGTCTCCCTGGCGCTGGGCAGACTTCACTGCCCACCCCCGGGTGCTGACTGTGGGGGACCGCA CGGGTGTGAAAATGGTTGACATTCAGGGCCCACCGGGCTGTGGGCTGCTGCTGTTCCGCGCTGGAGCAGA GGCTGCCTGCCAGAAAGGAGAGCGGGTGCTGCTTGCCCAGTACCTAGGGCAGCCAGGTCAGACCCCTCCA TCTCTGCATCTCATCTGCACCCAGTTCTCGATCTACCTGATGGATGAGCGCCTCCCCCTGGTGCCCATGC TTAAGTGGGACCATGGCTTGCCATCTGCTCCCCTGCTTGCCCGCCTGCTACCCCCAGCTAGCCCTGGCCA TCCACGGCCCCTGCTACTGGGGGGCCAGGGTGGGCAGCTGCAGCTGCTTCATATAACAGGAGAGGGAACT TCCATGCCACAGCTGGCGGGGCCTCCTCAGTCTCTGCCCTCCATCACCGAATCCCTCTCTGCCTTTCCCC TGCTGGAACCTAAGAAGCAGCAGCTGCTGCAGGAACGTCTGGAGGCACCAGTCATAGGTCTGGCTGCTGT CCCCCTCTGTGCCTCTGCCCCGGGGCTGTTGCTCTTCCAGCTCTCAGCAGCCGGGGATGTCTTCTACCAG CACCTACGGCTCCTGCAAGCCTCCAGTCCTAGAAAGGTGCCAGAGCAAGCAACAGCACCCTCAGTGGACC AGGTGTCCACGCCCTCTTGGACCCCTCAGGCCAGTGCCCGTTGCAGCCGCTGGTTGGAGGACCTGATGGA GTTGTCCCCCACTCGTCCATTGTGGGTCGCCCCGACCTTCTCCCATCGCCGTTTTCTGGGCCACATGGAG CGGCAGAAGAGCCAAGAGACCATGCCACAGAAGCTCCGAGCAGCCATGGCCAAGGGCCAGCTCCTGCGGC CTGGGGACCTGAGTACACTCCCCAGGGCAGAGCCACCCCCTGCACCTCAGTGCAGCCAACAGGATGAGCT CACTGAGCGCTTGACAGAAGCCTGGGAAGGCCGGGTGACTGCCTGGTGGAGGAGACATCGAGGTGAGACC TCCGAGACCCAGACCCAGTCCAAGCGGCCTAAGCGGCGGACTCAGTTGTCTAGCACCTTCTCCTCATTCA CAAGCTACCTGGACTCCCCAGATGCCAGCAGTGCCCCTCGTAGCCAAGACCTCTCTACCTCAGAGGCCCG TCTTCAGTCACCCAGGGTTCCACCCTCCCAGGAGTTGACACAGGAGGTGTGGGGCCAAGGTGTGAAGCGT GAACGCCGGCAGACCCTCCGGGATCACACGGACAAGCTGCCACTTAAGAGGGACACTCCAGGACCTGTGG CTACACCACCCTCCCAGGCCTCTAGCCTCCAGACCATGTCTTTCAGGCAGCAGACGCCTGTCCACTCTGG CTCCCAGCCTCCCCAGAAGAAGCCACGAATGGGCTTCTGAGTGGGGAAAGCCATATCTGAGAACCTAGCC TTCATCCTGGAGTGCTGCACTGAGCTCAGCCACTCCAGGGCAGAGCCTCCTCTGCCTCTGCCATGCAGAG AGCAGTGTGCCCATGGAAACGTGAGCAGTGTAGAACTAGGCTGAAGCAATGGGGAACCAGTCCACTTCGT GTATTTGATGGTAGGATATTCCCGAGAGCTGGCTGCAGAGGCTGCATCAGGATGGCTAATGCTGAGTCCC TGCAGTCCCAAGTCCCCCCAGTCTCCTGCCTTCTCCCACTGCCATTCTCTGGGCCACATGGAGCTGCAGA AAGAGCCAGGAGACCATGCCACAGAAGCTCCGAGCAGCCACGACCAAGGGCCAGCTCCTGTAGCTTGGGG ATCTGGCTACGTCTCCAGGGTAGAGCCACCCCCTGCACCTCAGTGCAGCAGGGTTGCTAGAAAGTAGCCT TAGCCTCCTGTCCTCAAAGATGCCCCAGCTCATGTGCTCCCCGAGGTAGCCCAGTGTGGGTCAGCTGTTG GAGGTTGATTGTGCCACAGTGTACATTGGTTCAGTAGGCATTGACGCTTTGACTCTGGGACTGAATAAAC CTGTCCACTGGCCATGAGCAGATGGTTATGTGGGCAGAGCAGATGTGAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:6811470 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811470 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7717 same embryo
	EMAGE:7718 same embryo
	EurExpress:euxassay_007477 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source