EMAGE mouse gene expression spatial database

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EMAGE:7726

Pramef8 PRAME family member 8 ( MGI:2140473)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007390. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007390_01	euxassay_007390_02	euxassay_007390_03	euxassay_007390_04

euxassay_007390_05	euxassay_007390_06	euxassay_007390_07	euxassay_007390_08	euxassay_007390_09

euxassay_007390_10	euxassay_007390_11	euxassay_007390_12	euxassay_007390_13	euxassay_007390_14

euxassay_007390_15	euxassay_007390_16	euxassay_007390_17	euxassay_007390_18	euxassay_007390_19

euxassay_007390_20	euxassay_007390_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7726_wholemount_strong.wlz

7726_wholemount_moderate.wlz

7726_wholemount_weak.wlz

7726_wholemount_possible.wlz

7726_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7726_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9047
Entity Detected:	Pramef8, PRAME family member 8 ( MGI:2140473)
Sequence:	sense strand is shown >T9047 TTTAGAGAAGCTCGGGAAGACACTCACCAGCACTTGGAGTCCGAGCTTGGACTGACTGCCTGGGCCTGCT GGGATTCTGAGTTCTGCCATCAGTTCTTGTTTCTGGATCCTTGACACTGAAGATCTGAAGGATGAGTGTC CAGGACCCACCCACACTCCTGCAGCTGTCAGTACAGAGACTACTGAGGGACGAGGCCCTGGCCATTTCTT CTCTGCAGTACCTTCCCAGGGCGCTTTTCCCACTGCTCTTCAAGGAAGCCTTCAATCACAGACAAACTAA TGTCCTGAGGGCCATGGTGGCAGTGTGGCCCTTTCCCTTCCTCCCTCTGGGGACCCTGATGAAGACTCCC CACCTGGAGATCTTGCAGGCTGTGCTGGATGGAGTAGACATGCTTTGGACACAGAAGGTTCTTCCCAGAA GGAGGAAGCTCCAAGTGCTGGACCTGAGGAATGTGTACCATGACTTCTGGGATGTTTGGGTTGGACCAGA GGATGGAGACCACTCAGCAGAGACTGTGTGTGAAAAGCAGATAGCAAAGCGCCTTCATCAATATGCACTG AGGCGACGTTTGAAGGTGGTCACTGACCTGTGCCTCAGGTTCCATTTGAATGAACACCAAGCATACTTGT TACAGTGGGCCCAGCAGAGAAGAAGTTCCATACGGCTATGCTGTGTGAAGATGCAGATTTGGGCTTTGCC TGTGTACACTGTCAGGAAGGTCTTGATGGTGTTCCAGCCAGACAGCATCCAAGAATTGGAACTAAATACA GGTTGGAGCCTGTCCACTCTGGTGCATTTTGCATCTTACCTGGACCAGATGAGAAACCTTCAAAAACTCC TTTTAACACGGATTCACAAGAACACCTTCAAGGTTCTAAATACCTCCTCGGACATCCAGAAGTGTATCAC CAAGTTTGTCTCTCAGTTCTCCAAACTCAACTCTCTCCAGCATCTCTCCATGAATGGCATCTACTTTTCC AGTGAGCACATGAAACAGTTGTTCAGGTACCTGAAGACCCCGTTGGAGACTCTGTCCATCACCATGTGCA AGCTTTCACATTTAGACTTGAATTCCTTGTCCCAGAGTCAGAGCCTCCATCAGCTCAAACAGCTGAACCT GAGACTTGTGAAGTTGATTGACTTACGTCCTGTGCCTTTCCACGACCTCCTCAGGAGTGTTGCAGGCACC CTGCAGACCCTAGAACTGGAGGGCTGTTGGATGGTGGACTCCCAGCTCACTGCCCTCCTGCCTGCCCTGA GCCAGTGTTCCCAGCTCACCAGGGTCAACTTCTATGACAATGACATCTCCATGGCTGTTCTAAAGGACCT TCTGTATCACACAGCCAGTCTGAGCCAGCTGACCCAGGAGCTGTATCCTGCCCCTCTGGAGTGCTATGAT GACACGGGTGATGTCCTTATGGGAAGATTTGTCCAACTTTGTCCTGAGCTTATGGAGACACTCATTACTG TAAGGCAGCCCAAGAATGTCTCCTTTGCTACGTATATCTGCCATGAATGTTGCCAGCGCTGTGTCTATGG CCTGGAGACAAAACTTTGTCGTTGTCGGCAGTAAATAGGGGCAGGTTGTTTTTTGATACTGAGAAATGAA AGTCTAGGAACAGGTCTTGCATCAAGAGAACCCAAAGCACATGGCCACACACTGCTCATTATCTGGGAAC CCAAATGGTGTGCTGGAGGGTTAGACCACAAAGACTGAGCTTCTAGAGAACAATGGGACCCAGGACAGAT GTTTGTGGAATCAAAAGACAATCCTTCATTTCTTTTCTTTTGTTCATTCTTCATTGTTTTTTTGTTTTCT TTTAAGATTTATTTATTTTATATCTGGGTGGTTTACCTGCATATATGTATCCTTGGTGCCTATAGAGTTC AGATGTGGGCATTGGATCCCCTGGAACTGGAGTTTTGGATGGCTGTGACCCATCATATGAGTCTTCTGCA AGAACAAGTGCTCTTGCCTGCTGAACCATCTCTCCAGCCCCAGTTGAGCACTTTCTAAATAAATGAGTTC TGTCAGGTCATGATAATGAATGACTTTCTCCTTCTCACATGGTAGTAAAATGGCCAGATTTTAGAACCTG GATGTTCTGGTGTCCTTCTCACCAGATTTTTGGAGCTTGGTTTCAGTGTTGTTCTTTTCCTGTGGCTTGG CTGGAAAGATCCTGTGCTGTCTGACCTCTCTAGACATCTGTAATGGCAAACCTGGTTGTCAGCCTGCCCA CTTCTGTAACAACTAGAACCCACTTGATGAAACAGCACATGCCCTAAATGGTGACCATGCATTCTCTTGG CAGCATTGGTAAAGAGAGAGAAGAAGGAAGCTGTGCTGCCGAGTTTGTCCTACTTCTTTGGGCAGTATTC ATTTTCTTCAGGATTTTAACATAGCCTGAAAGCCAGCAGCTCTCTAGGAGCCTAGGATTGCAGCACCAGA TTGGACTGCAGAGACTTCCAGCTTTACGAATGAACTCCCAGATCCTTGGCCTTTTCATTGTGAGCCAGCC ATTGTCAGACTGCGCAGACCACAGCCGGTAAGCCAGTCTAGTAAACCTCATATGTTCCTGATACACACGG AACTGTCAGTTCTGCTCCTTTAGCCTTTAGAGAACCCTGACCACTACAGCATCAAGGTCAAGTGAAAGCC GTCAGATGTCTTCCCTCCTTTACCTTTGCCTGGGCATCTGTTATGTCTCACTTGTGTGCTCATTGCTTTA GTATACAAAATGTCAATGTAAAATGAGCAATGCATTCTCCTCTGAGGGGTTTGGGGGAATGGTAGAGGAA TCCAATCTAGTACACTTAGACTAGGAGGTATTATGGACATATCAAAGATGGCTGAAGTGAAGTCAAAGAT AACAAAGAGCAAAGAAAATGGAGGCTAAGCCACTTTGCTTTATCACTCTTTATTTTTTTCTCTCTCTTTC ATGGTCACATATTGCCCAGGCTGGCCTTGAAATCTCCTGCCTCCACCTTCTAAATGCTAAGATTACTGAA GTATGGCACCTTGGCTAGGTTGTAGTCCACATGTATGTAAGACTCTATTTTTCAGGGCTTCTAGCCTCCG GCTGTCCCTTTGAAAACAAAGGTCTCCAGTAGATGTCCCTGTCCCCTAAAGGATTGACAGGCCTTAAGAA CTGCATTATAGGTGTCCAGGAGTAAACTTTTATTTTGTTAATAAGTATCTAAGCCTAGCCATTGCGATCC TCTGTATAATCTGGTTGCAATAAAGAATTTTGGTTCATAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816019 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816019 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7730 same embryo
	EMAGE:7728 same embryo
	EMAGE:7727 same embryo
	EMAGE:7729 same embryo
	EurExpress:euxassay_007390 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source