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EMAGE:7734

Arhgef6 Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 ( MGI:1920591)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7734 EMAGE:7734 EMAGE:7734 EMAGE:7734 EMAGE:7734
"Pseudo-wholemount" of euxassay_007721. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007721_01 euxassay_007721_02 euxassay_007721_03 euxassay_007721_04
EMAGE:7734 EMAGE:7734 EMAGE:7734 EMAGE:7734 EMAGE:7734
euxassay_007721_05 euxassay_007721_06 euxassay_007721_07 euxassay_007721_08 euxassay_007721_09
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euxassay_007721_10 euxassay_007721_11 euxassay_007721_12 euxassay_007721_13 euxassay_007721_14
EMAGE:7734 EMAGE:7734 EMAGE:7734 EMAGE:7734 EMAGE:7734
euxassay_007721_15 euxassay_007721_16 euxassay_007721_17 euxassay_007721_18 euxassay_007721_19
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euxassay_007721_20 euxassay_007721_21 euxassay_007721_22 euxassay_007721_23 euxassay_007721_24
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euxassay_007721_25 euxassay_007721_26

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7734Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7734_wholemount_strong.wlz
7734_wholemount_moderate.wlz
7734_wholemount_weak.wlz
7734_wholemount_possible.wlz
7734_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7734_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35947
Entity Detected:Arhgef6, Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6 ( MGI:1920591)
Sequence:sense strand is shown

>T35947
CCTCGATTCTCCAGTAACCATCACTGTGCAGCTGAGCACAGTGCCTTCAGCACTCCTTAAAGTGGCCAGC
TGAATGGTTTCACTGTGCATTTGAGCTTTGTGCTTTGTGGTGTTGGGATGCTCTCTCTTTCTTCCTCTCT
CCCTTCCCCTCCCCCTTCCCCCTCCACTGTTTGATTCAGAGTTTATTGCTTGGTTATCAGTTGATTGACA
CTTGTTTTAACAGGGGGAAATAGCCTGACGTGGTGGCCAAAATGGCCCCAATACTTTGCTATGCCCAACA
GCCCTTTACTACCTAATTCGCTGGAGGCTAAGCTTCTCAGGCTATGTCTAGAGTAGGTGAAAATGAACAC
CTTTGCCCAGTGACCCAGTTGCACTTCCCTTTACTATCATATGGCCCCCTTCAGTACTTTAAGAACTTAT
TGCCCATGTTGTCTGTTGAGATCAAGAACAGTGTAGTGCCCTTATGCCCTCTGCCCTCTGTGCTGTTGTC
CATTTAAAGTCAGTGTGGTAGGAACTCCAACCATCCACACCAGCACCAAGTCTCTCAGCAAATTGCTTCA
GGCAGTCCTCCGTCAGCAGCAGGGCAAAGCAGCTTGATGGAGTACTTATCCCTAGTATAGCGTAGCTTTA
CAGCCCAGCCCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 101519. Forward Primer - name:101519_F_cDNA_Arhgef6, sequence:CCTCGATTCTCCAGTAACCATC; Reverse Primer - name:101519_N_SP6_cDNA_Arhgef6, sequence:GAGGGCTGGGCTGTAAAGCTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7735 same embryo
 EMAGE:7732 same embryo
 EMAGE:7731 same embryo
 EMAGE:7733 same embryo
 EurExpress:euxassay_007721 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS