EMAGE mouse gene expression spatial database

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EMAGE:7746

Dclk3 doublecortin-like kinase 3 ( MGI:3039580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007494. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007494_01	euxassay_007494_02	euxassay_007494_03	euxassay_007494_04

euxassay_007494_05	euxassay_007494_06	euxassay_007494_07	euxassay_007494_08	euxassay_007494_09

euxassay_007494_10	euxassay_007494_11	euxassay_007494_12	euxassay_007494_13	euxassay_007494_14

euxassay_007494_15	euxassay_007494_16	euxassay_007494_17	euxassay_007494_18	euxassay_007494_19

euxassay_007494_20	euxassay_007494_21	euxassay_007494_22	euxassay_007494_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7746_wholemount_strong.wlz

7746_wholemount_moderate.wlz

7746_wholemount_weak.wlz

7746_wholemount_possible.wlz

7746_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7746_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9054
Entity Detected:	Dclk3, doublecortin-like kinase 3 ( MGI:3039580)
Sequence:	sense strand is shown >T9054 AGCTGCCAGGCCCCTGGTTACCTGCGGGACGAGGACCTCTGGAGAAGCCAGTTCTGGGGCCACGTGGTGC CGTCATGCCGCTGTTCAGCCCTCAGAGCAGCCTCCACTCAGTCCGCGCAGAGCACAGCCCACTGAAGCCC AGGGTGGTGACGGTGGTGAAGCTGGGTGGGCAGCCCCTCCGTAAGGCCACCCTGCTCCTCAACCGGCGCT CAGTGCAGACCTTTGAGCAGCTCCTATCAGACATCTCCGAAGCCTTGGGCTTCCCACGCTGGAAGAACGA CCGTGTGCGGAAGCTGTTCACCCTCAAGGGCAGGGAGGTGAAGAGTGTGTCTGACTTCTTCCGGGAGGGT GATGCTTTCATAGCTATGGGCAAAGAGCCGCTGACATTGAAGAGTATCCAGTTGGCCATGGAGGAGCTGT ATCCTAAGAACCGGGCTCTTGCCCTGGCCCCTCACAGTAGAGTCCCCTCCCCAAGGCTGAGAAGCAGACT TCCCAGCAAGCTTCTGAAAGGAAGTCACCGCTGTGGGGAGGCAGGAAGCTATAGCGCGGAAATGGAGAGT AAGGCAGTCTCTAGGCATCAGGGCAAGACTTCCACAGTGCTGGCCCCAGAAGACAAGGCGAGGGCCCAGA AGTGGGTAAGAGGGAAACAGGAGTCAGAACCTGGTGGCCCGCCTTCACCCGGGGCAGCCACTCAGGAGGA GACTCATGCAAGTGGAGAGAAACATCTGGGGGTGGAGATCGAAAAGACCTCCGGGGAGATTGTCAGATGT GAGAAGTGTAAGAGAGAAAGAGAGCTGCAGTTGGGCCTGCAGAGGGAGCCGTGCCCGCTGGGAACCAGTG AGCTGGACCTGGGGAGAGCTCAGAAGAGGGATTCCGAGAAGTTGGTGAGGACCAAGAGCTGCAGGAGGCC TTCTGAGGCAAAATCTACAGATGGAGAGGAAGGGTGGAAGGGTGACAGCCATCGGGGCAGTCCCAGGGAC CCCCCTCAGGAACTGAGGAGGCCCAACAGCAACTCAGACAAGAAAGAGATCAGAGGCTCAGAAAGTCAGG ACAGTCATCCTCAGGGGGCACCCAAGGCCCAGAAGGACCTCGTGGAAGGGCCACCAGCTGTAGAGGAGGG GCCGATAGACATGAGGAGAGAGGACCGGCACACATGCAGGAGCAAGCATGCCGCCTGGCTCCGGAGAGAG CAGCAGGCCGAACCCCCACAGCTCCCCAGAACCCGAGGGGAGGAGAAGCAAGCAGAGCACGAGAAGAAGC CAGGCGGCTTAGGAGAGAGGAGGGCGCCAGAGAAGGAGTCTAAGAGGAAGCTAGAAGAGAAGAGGCCAGA ACGACCCAGTGGCCGGAAGCCGAGGCCCAAGGGCATCATCTCAGCGGATGTGGAGAAGCACTATGACATA GGTGGGGTCATTGGGGATGGCAACTTTGCCACCGTGAAGGAATGCAGGCACCGAGAGACCAAGCAGGCTT ACGCCATGAAGATGATTGACAAGTCCCAGCTGAAGGGTAAGGAGGACATTGTCGACAGTGAGATTTTAAT CATCCAGAGTCTCTCTCATCCCAACATTGTGAAACTGCACGAGGTCTACGAGACGGAGGCGGAGATCTAC CTGATCATGGAGTATGTGCAGGGAGGGGACCTTTTTGATGCCATCGTTGAAAATGTGAAGTTTCCAGAGC CCGAGGCTGCAGTTATGATCACAGACTTGTGTAAGGCCCTCGTCCACATGCACGACAAGAATATCGTCCA CCGGGACGTGAAACCAGAAAACCTCCTGGTTCAGCGAAATGAAGACAAGTCTATCACCTTGAAGCTGGCT GATTTTGGCTTGGCCAAATATGTGGTGAGGCCTATATTTACTGTGTGTGGGACGCCAACATATGTAGCTC CTGAAATTCTTTCTGAGAAAGGTTACGGCCTGGAGGTGGACATGTGGGCGGCAGGTGTGATCCTATACAT CCTCTTGTGTGGCTTCCCCCCTTTCCGAAGTCCTGAGAGGGACCAAGACGAGCTCTTCAACATCATCCAA GTGGGCCAGTTTGAGTTCCTCTCTCCTTACTGGGACAACATTTCTGATGCTGCCAAAGATCTGGTGAGAA ATTTGCTGGAGGTGGACCCTAAGAAGCGGTACACGGCCGAACAGGTCCTACAGCATCCCTGGATTGAGAT GGTTGGGCATACCAACACAGGGAACTCACAGAAGGAGGAGTCCCCCAACAGTTAGGGTCACTTCCAGAGT CAGCACAAGAAGGTTGCAGAGCAGATGCCATAATCCTGCCTGGGACATCTGTCCAGCCTGGTCTCCTTGA GGACAGAGAAGAGAGGGGACGTCTAGTCTACAAGCAAGGCTGTGCAAGACCCTTCTCCACAGAATTAGGG AAGGAGAAGAGCCACCTGGCATATTTTAAAGAGTGTTTGAAAGCAATGTTCTAATGTTGAATGATGTATG ATATTTTTAGATTTGTATATCTGAAGTCTTTAATACCTTTTGGGTAAGAATTGCTATGAATGAGGAATTT TGATAATAATGACAGATTCTGTTTTCTCCTTGTATTCACATCCATGGTAGGCTACAGGAGTGTGCTTCAC ATCGCTCAAGCTCCCCCACACAATGAATGAGGTGATTCCTGGCTTTTTTAAAAATCTCAATAAAACACAA TGAATAACAGAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816292 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816292 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7744 same embryo
	EMAGE:7742 same embryo
	EMAGE:7741 same embryo
	EMAGE:7743 same embryo
	EMAGE:7745 same embryo
	EurExpress:euxassay_007494 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source