EMAGE mouse gene expression spatial database

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EMAGE:7765

Man1a2 mannosidase, alpha, class 1A, member 2 ( MGI:104676)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007471. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007471_01	euxassay_007471_02	euxassay_007471_03	euxassay_007471_04

euxassay_007471_05	euxassay_007471_06	euxassay_007471_07	euxassay_007471_08	euxassay_007471_09

euxassay_007471_10	euxassay_007471_11	euxassay_007471_12	euxassay_007471_13	euxassay_007471_14

euxassay_007471_15	euxassay_007471_16	euxassay_007471_17	euxassay_007471_18	euxassay_007471_19

euxassay_007471_20	euxassay_007471_21	euxassay_007471_22	euxassay_007471_23	euxassay_007471_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7765_wholemount_strong.wlz

7765_wholemount_moderate.wlz

7765_wholemount_weak.wlz

7765_wholemount_possible.wlz

7765_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7765_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9026
Entity Detected:	Man1a2, mannosidase, alpha, class 1A, member 2 ( MGI:104676)
Sequence:	sense strand is shown >T9026 CCAAGAGCCTAAACGATGACTACCCCAGCGCTGCTGCCCCTCTCTGGTCGTAGGATACCACCTTTGAACC TGGGGCCGCCCTCCTTCCCACATCACAGGGCTACCTTGAGACTCTCCGAGAAGTTTATCCTTCTCCTCAT CCTTAGTGCCTTCATCACCCTGTGTTTTGGGGCATTCTTCTTCCTTCCAGACTCTTCAAAACACAAACGC TTTGATCTGGGCTTAGAAGATGTGTTAATTCCTCACGTAGATGCCGGCAAAGGAGCTAAAAACCCCGGCG TCTTCCTGATCCATGGACCCGACGAACACAGACACAGGGAAGAAGAAGAGCGTCTGAGAAATAAGATTAG AGCTGACCATGAGAAAGCCCTGGAAGAAGCAAAAGAAAAATTAAGAAAGTCAAGAGAGGAAATCCGTGCA GAAATTCAGACAGAGAAAAACAAAGTAGCCCAAGCAATGAAGACAAAAGAGACCAGGGTACTGCCGCCTG TCCCTGTCCCACAACGTGTAGGGGTCAGTGGTGGGGATCCAGAAGACATGGAGATCAAGAAGAAAAGAGA CAAAATTAAAGAGATGATGAAACATGCCTGGGATAATTACAGAACATACGGATGGGGACATAATGAACTA AGGCCTATTGCAAGGAAAGGCCATTCCACTAACATATTCGGAAGCTCACAGATGGGTGCCACCATAGTGG ATGCTTTGGATACCCTTTATATCATGGGGCTTCATGATGAATTCATGGATGGGCAAAGATGGATTGAAGA AAACCTTGATTTCAGTGTGAATTCAGAAGTGTCTGTCTTTGAAGTTAACATTCGCTTTATTGGAGGGCTC CTCGCTGCATATTACCTGTCAGGAGAGGAAATATTCAAGACTAAAGCAGTGCAGTTGGCTGAGAAACTCC TTCCTGCCTTTAACACACCTACTGGGATTCCCTGGGCAATGGTGAACCTGAAAAGTGGAGTAGGTCGAAA CTGGGGCTGGGCGTCTGCAGGCAGCAGCATCCTGGCTGAGTTCGGCACCCTGCACATGGAGTTTGTGCAC CTCAGCTACTTGACCGGTGACTTGACTTACTATAATAAGGTCATGCACATTCGGAAACTACTGCAGAAAA TGGAACGCCCAAATGGTCTTTATCCAAATTATTTAAACCCAAGAACAGGGCGCTGGGGTCAGTATCACAC ATCAGTTGGTGGTCTGGGAGATAGTTTTTATGAATACTTACTGAAAGCATGGCTGATGTCAGATAAAACA GACCACGAGGCAAGAAGGATGTATGACGATGCTGTTGAGGCTATAGAAAAACATCTTATTAAGAAGTCCC GAGGAGGTCTGGTTTTTATTGGAGAATGGAAGAATGGACACTTGGAAAGGAAGATGGGGCACTTGGCCTG CTTTGCTGGGGGAATGTTTGCCCTTGGAGCAGATGGTTCCAGAAAGGATAAAGCTGGCCACTACTTAGAA CTAGGGGCAGAAATTGCACGAACATGTCATGAGTCATATGACAGAACTGCATTGAAACTAGGTCCGGAGT CATTCAAGTTTGATGGTGCAGTGGAAGCCGTGGCTGTGCGGCAGGCTGAAAAGTATTACATCCTTCGTCC AGAAGTAATTGAAACCTATTGGTATCTATGGCGATTTACCCACGACCCAAGATACAGGCAGTGGGGCTGG GAAGCAGCACTGGCTATTGAGAAGTCGTGCCGGGTCAGCGGTGGGTTTTCTGGTGTCAAGGATGTATACG CCCCGACCCCTGTGCATGACGACGTGCAGCAGAGCTTTTTTCTTGCTGAAACATTAAAATACTTGTACCT GCTGTTCTCTGGCGATGACCTTCTACCTTTAGACCACTGGGTGTTTAACACAGAGGCGCACCCTCTGCCG GTGTTGCGCTTAGCCAACAGCACTCTTTCAGGTAATCCTGCTGTCCGATGAGCACAGCCCCAGAAGGACC ATTCTTACCTGTGTTTTGTTTACATGGACCACTACAGAGACTGTCTGCAGAGGAGAGGCGGCTGTGGGAA ACTGGGACTCTTATGTCAGTATGCCGGATGAAACTCTTCCCTGCAAGACTTTTCACTTGTACATATATCA ACTTTGAAATTATTCCATTTTATACCTGACCAAAACAAGTTCTGGTACATGTAGGACAGAGACCTGATGT GTTTTGATTCTTACTGAGATGGCCACATGAGAAACAATGTCTTATTCTATACTGTCAGTGCAAGTCAAGA CCATAGAGCACCTTACAGGAGCTAGAGATGGCTTTTTGAACCAGTTATACATTTGTTTTCTCCCACAGTG GAGCAGCTCTCAAATCAAATATAACACATTGTGTAGCCCCTTTCCTCCATTTTAACAATGGAACAAACCG AATGAGCAGGAACCGAGGAACATGTAACTACATTGTAGCAAGAAGACTTGGGAAAGAAACAAACAGATAC TGGCCCTGTCTGAATTTTCCAGCTCCTCATTGGATTTTTACCAGACTGGCAACCATTTCATTCCCAACCT AGGCATTGAAATCTGTTTAGATTGAGTTTTTCTCTGAGGGGCATGATCTCATGAAGAATATTTTTCAAGT CGTTGTCTCCATATAATATGCTGAACCTGGTATTTATCATAACTGCTGCTTATGAGCCGGAGTGTGGGAG TGTGCATGTAGCTCACGCTAGCATTGGGAGCATGATGGAAGGAGGGAAGGTGCCAGATTGTTGCCTTGAA ATCTGTTTTAGAGAAATCCTTAATTATTCTTCTGAATCAGCAACTATATCAAGCAAACATTTTCTGCTTT AATCTCATAACACACTTAATAGAGTCATTCACCTTACAACTCCATGTGGTTCGGCAAAATTGACTCTACT GTTATGCAGCTCTGTATTTGCTAGAACAAACCTAATCGCTTTTACAACTTACTAAAGCAAAGGCAAAACA TAGCAATGTCCCTTGAGTAGGGTCAGGTCCTTCACTCATTACCTCACACGTGTGATCTGTTCACAAAGAG TCTATGTTTTTAAGTTTCTCCAAGATATTATCCATCCAAAGAATTACTAGAATAGCTTCGGAATAATTTT CAGTGGCAAATGTGTGACGTAAAGAATCATACTCACTAAAATTGGCATGAACCTAAGGGACTTACAGACC TGCACTGGTTATTTTTGTTGTTGATACTGCTCTATATTTTGGCAGAAAAAAGGGTAAATTTTTATCAAGA ACTATAGTTCTTATTATAATATAAGATAAATAGTAATGCTATAAATTTTCTGTCCCTTGAATTTCTGTGA TAATTCAAGACACTTTCTCTCTTTTATTTCATACATACATCATCTATTGATGACAATAAATTTGAGAACG CTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6813258 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6813258 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7764 same embryo
	EMAGE:7766 same embryo
	EMAGE:7767 same embryo
	EMAGE:7763 same embryo
	EMAGE:7768 same embryo
	EurExpress:euxassay_007471 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source