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EMAGE:7781

Tomm22 translocase of outer mitochondrial membrane 22 homolog (yeast) ( MGI:2450248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781
"Pseudo-wholemount" of euxassay_007431. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007431_01 euxassay_007431_02 euxassay_007431_03 euxassay_007431_04
EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781
euxassay_007431_05 euxassay_007431_06 euxassay_007431_07 euxassay_007431_08 euxassay_007431_09
EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781
euxassay_007431_10 euxassay_007431_11 euxassay_007431_12 euxassay_007431_13 euxassay_007431_14
EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781
euxassay_007431_15 euxassay_007431_16 euxassay_007431_17 euxassay_007431_18 euxassay_007431_19
EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781 EMAGE:7781
euxassay_007431_20 euxassay_007431_21 euxassay_007431_22 euxassay_007431_23 euxassay_007431_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7781Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7781_wholemount_strong.wlz
7781_wholemount_moderate.wlz
7781_wholemount_weak.wlz
7781_wholemount_possible.wlz
7781_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7781_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9079
Entity Detected:Tomm22, translocase of outer mitochondrial membrane 22 homolog (yeast) ( MGI:2450248)
Sequence:sense strand is shown

>T9079
ACAGCCATGGCCGCCGCCGTCGCTGCAGCCGGCGCTGGGGAGCCTCTGTCCCCCGAGGAATTACTCCCGA
AAGCCGAGGCAGAGAAAGCCGAGGAGGAGCTGGAAGAAGACGACGACGACGAGCTAGACGAGACCCTGTC
GGAGAGACTCTGGGGTCTGACGGAGATGTTTCCCGAGAGGGTCCGGTCAGCCGCCGGAGCCACCTTTGAC
CTCTCGCTCTTCGTGGCTCAGAAGATGTACAGATTTTCCAGGGCAGCTTTGTGGATTGGGACCACTTCCT
TCATGATCCTGGTTCTTCCCGTTGTCTTTGAGACAGAAAAGTTGCAGATGGAGCAACAGCAGCAACTGCA
GCAACGGCAGATACTTTTAGGGCCTAACACAGGGCTGTCAGGAGGAATGCCAGGGGCTCTACCTCCACTT
CCTGGAAAGATGTAGATTCTTCCTTCGGTATTTGTCCTTGTGGGAGAACTTTGAAATTCATTTGTGTTTG
AACTGCTGATTGTTGGAACTTTTTATTCTTTTTTTTAACCTTGGCACATGGATCTATTTGAACATAGTGG
GAACTTTCTCTGTTTCTCATGGAGAGACCCCAGGTACACCTGTGCCCTACATAATGAAGTCCCTCACTTA
CTTCTTCCTGTTCTCACTCCAATGCTGGAGTACAGCCATGTGGAAGGTTAGCCTACTCTCTAATAAACTG
CTCCTAAGGAAGTTCTTACATGCCCATTGTGGGAAAGAAGTAAGTACCCCTGACCATAAGGACTTTTTTT
TAATGGAAATGTCTAGGACATGTAAGCACCGTGGCACTGACAAAATTCATTATACCTGAGTACAGAAGTC
ACCAGGAATCCCAGAGAGAAGGGAGGCTGGCTGTAGCTTTAGCCAGAGGGGGACTGGGCAGAGCAGGCAC
TCATTTCCTCTGCAGAGTTTGGCTCTTAGACCTGCTTATGTATAAAAAAGAAGTCAGCGCTCACTTTTTG
TATTTAAATATTAAAAGAACTCGAACTGAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6819748 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6819748 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7785 same embryo
 EMAGE:7784 same embryo
 EMAGE:7782 same embryo
 EMAGE:7783 same embryo
 EurExpress:euxassay_007431 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS