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EMAGE:7798

Klf4 Kruppel-like factor 4 (gut) ( MGI:1342287)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798
"Pseudo-wholemount" of euxassay_005264. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005264_01 euxassay_005264_02 euxassay_005264_03 euxassay_005264_04
EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798
euxassay_005264_05 euxassay_005264_06 euxassay_005264_07 euxassay_005264_08 euxassay_005264_09
EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798
euxassay_005264_10 euxassay_005264_11 euxassay_005264_12 euxassay_005264_13 euxassay_005264_14
EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798
euxassay_005264_15 euxassay_005264_16 euxassay_005264_17 euxassay_005264_18 euxassay_005264_19
EMAGE:7798 EMAGE:7798 EMAGE:7798 EMAGE:7798
euxassay_005264_20 euxassay_005264_21 euxassay_005264_22 euxassay_005264_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7798Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7798_wholemount_strong.wlz
7798_wholemount_moderate.wlz
7798_wholemount_weak.wlz
7798_wholemount_possible.wlz
7798_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7798_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 02 03 04 05 06 18 20 21 22 23 moderate expression: see section 07 08 19 weak expression: see section 17
forelimb
moderate moderate
regionalmoderate expression: see section 23
arm
strong strong
regionalstrong expression: see section 01 02 03
hand
moderate moderate
regionalmoderate expression: see section 22
hindlimb
strong strong
regionalstrong expression: see section 03 moderate expression: see section 23
foot
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 05 06 19 20 21 22 weak expression: see section 04
lower leg
moderate moderate
regionalmoderate expression: see section 22
fibula
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 21
tibia
strong strong
regionalstrong expression: see section 01 02 moderate expression: see section 21
femur
strong strong
regionalstrong expression: see section 04 moderate expression: see section 20 21
upper leg rest of mesenchyme
strong strong
regionalstrong expression: see section 04
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 21 22 23 moderate expression: see section 08 09 10 11 12 13 15 16 17 18 19 20
vibrissa
moderate moderate
regionalmoderate expression: see section 04 05 06 18 19 20
esophagus
strong strong
regionalstrong expression: see section 12
mandible
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22
lower jaw molar
strong strong
regionalstrong expression: see section 15
maxilla
strong strong
regionalstrong expression: see section 04 06 07 08 09 10 14 15 16 17 18 19 20
palatal shelf
strong strong
regionalstrong expression: see section 09 11 12 14
upper jaw molar
strong strong
regionalstrong expression: see section 15
bladder
strong strong
regionalstrong expression: see section 11 12 13 14 15
genital tubercle of male
strong strong
regionalstrong expression: see section 11 12 13 14
cranium
strong strong
regionalstrong expression: see section 01 02 03 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 04 05 19 20 21 22
clavicle
strong strong
regionalstrong expression: see section 08 09 16 17 18
extraembryonic component
moderate moderate
regionalmoderate expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2473
Entity Detected:Klf4, Kruppel-like factor 4 (gut) ( MGI:1342287)
Sequence:sense strand is shown

>T2473
TGGCCTCGAGCCAGATTCGGCACGAGGAAGAAAAAAATCACAGAACAGATGGGGTCTGATACTGGATGGA
TCTTCTATCATTCCAATACCAAATCCAACTTGAACATGCCCGGACTTACAAAATGCCAAGGGGTGACTGG
AAGTTTGTGGATATCAGGGTATACACTAAATCAGTGAGCTTGGGGGGAGGGAAGACCAGGATTCCCTTGA
ATTGTGTTTCGATGATGCAATACACACGTAAAGATCACCTTGTATGCTCTTTGCCTTCTTAAAAAAAAAA
AAAGCCATTATTGTGTCGGAGGAAGAGGAAGCGATTCAGGTACAGAACATGTTCTAACAGCCTAAATGAT
GGTGCTTGGTGAGTCGTGGTTCTAAAGGTACCAAACGGGGGAGCCAAAGTTCTCCAACTGCTGCATACTT
TTGACAAGGAAAATCTAGTTTTGTCTTCCGATCTACATTGATGACCTAAGCCAGGTAAATAAGCCTGGTT
TATTTCTGTAACATTTTTATGCAGACAGTCTGTTATGCACTGTGGTTTCAGATGTGCAATAATTTGTACA
AT
Notes:The probe template was PCR amplified from IMAGE:1248344 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1248344 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7799 same embryo
 EMAGE:7802 same embryo
 EMAGE:7801 same embryo
 EMAGE:7800 same embryo
 EurExpress:euxassay_005264 same experiment
 MGI:4825783 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS