EMAGE mouse gene expression spatial database

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EMAGE:7901

Farsa phenylalanyl-tRNA synthetase, alpha subunit ( MGI:1913840)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012957. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012957_01	euxassay_012957_02	euxassay_012957_03	euxassay_012957_04

euxassay_012957_05	euxassay_012957_06	euxassay_012957_07	euxassay_012957_08	euxassay_012957_09

euxassay_012957_10	euxassay_012957_11	euxassay_012957_12	euxassay_012957_13	euxassay_012957_14

euxassay_012957_15	euxassay_012957_16	euxassay_012957_17	euxassay_012957_18	euxassay_012957_19

euxassay_012957_20	euxassay_012957_21	euxassay_012957_22	euxassay_012957_23	euxassay_012957_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7901_wholemount_strong.wlz

7901_wholemount_moderate.wlz

7901_wholemount_weak.wlz

7901_wholemount_possible.wlz

7901_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7901_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 15 16

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 07 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 07 08 17 18

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 17 18 19

ventral grey horn

strong

regional

strong expression: see section 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38860
Entity Detected:	Farsa, phenylalanyl-tRNA synthetase, alpha subunit ( MGI:1913840)
Sequence:	sense strand is shown >T38860 AGGATTTCCCTGCCCTACTAAGGCTAGGCTTAAAGGTATATGTCACAATGCATTTTTATTTCATTTTATT CAGAATTCTACTGGCTGCCCTGAAACTCATTATGTAGACCAGGCTGGCCTCAGACTCACAAAGATCCACT GTCTTCTGCCTCCCTAGTGTTTGTACTATAGGCATGGGCCACCATGCCTAGCATGTCCCAGGGTTGTACT ACAGGCATGGGCCCACCATGCCTAGCATGTCCCAGGGTTGTACTACAGGCATGGGCCACCACGCCAGCAT TTCCCAGGGTTTTGTTTTGTTTTTTTTTTTCCCCCTGGGGACAGGGTTTCTTGGTAGCCGTGGCTGTCCT GAAACTCCCTGTAGACAAGGCTGACCTGAAAATTAGAGATTCACCCGCTTCTGTCTCTGCCTCCTGAGTG CTGGGATAAAAGGCATGCATCATCACTGCCTGGCCTCATGTCCAAGATTTTTAATGCCACCTAAGGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 177511. Forward Primer - name:177511_F_cDNA_Farsla, sequence:AGGATTTCCCTGCCCTACTAAG; Reverse Primer - name:177511_N_SP6_cDNA_Farsla, sequence:GCCCTTAGGTGGCATTAAAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7899 same embryo
	EMAGE:7902 same embryo
	EMAGE:7898 same embryo
	EMAGE:7897 same embryo
	EMAGE:7900 same embryo
	EurExpress:euxassay_012957 same experiment
	MGI:4824779 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source