EMAGE mouse gene expression spatial database

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EMAGE:8043

9430020K01Rik RIKEN cDNA 9430020K01 gene ( MGI:2685174)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015963. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015963_01	euxassay_015963_02	euxassay_015963_03	euxassay_015963_04

euxassay_015963_05	euxassay_015963_06	euxassay_015963_07	euxassay_015963_08	euxassay_015963_09

euxassay_015963_10	euxassay_015963_11	euxassay_015963_12	euxassay_015963_13	euxassay_015963_14

euxassay_015963_15	euxassay_015963_16	euxassay_015963_17	euxassay_015963_18	euxassay_015963_19

euxassay_015963_20	euxassay_015963_21	euxassay_015963_22	euxassay_015963_23	euxassay_015963_24

euxassay_015963_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8043_wholemount_strong.wlz

8043_wholemount_moderate.wlz

8043_wholemount_weak.wlz

8043_wholemount_possible.wlz

8043_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8043_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 06 07 17 18 19 21 22 23 24 25

diencephalon meninges

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon meninges

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

hindbrain meninges

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord meninges

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

tail mesenchyme

weak

regional

weak expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39585
Entity Detected:	9430020K01Rik, RIKEN cDNA 9430020K01 gene ( MGI:2685174)
Sequence:	sense strand is shown >T39585 CACAAAAGCAGCCCCTTTATACTGTCTGTCAGAACCAAGAGGAAGTCAAGAACTCACCAAATTCGGCGAT GCTGTAGGGTCTGTGCAGCTGGGCAGAGAGACTCCCACACAGGTGGGTAATGGTGGGGACACAGAGGTCC TACCCTGTGTCCTTCTGCCACTGGCTGACAAATATCGAGGCCACTCAACACCAGACTTTCGGTCTTTAGA GCTCACACTGGGACAAGAACAGAATGCCTATAAATTAGAGTGTCTGGATTTAGAGAACACCGTGGAAGTC CTTCCAAGTGAGTCTCTGCAGGAAAGGGCAGAGAGGATCCTGGGCATAGAGGTGGCCGTGGAGTCCCTTC TGCCAAGTGCCAGGAGAACAGAACAAAGCCAGCTTCCTGAGCCTGATGCAAGTGCCTGCAACCCAAGTTC ATCCAGAGAGGACTCATCACACAGCTTGGCACTACCAGTGGGGCCCAAAGTGGCCACTGACGCCTTCTAT GGCAGGAGGAAGTGTGGCTGGACTGAGAGTCCTCTTTTTGTAGGAGAAAGGGCCCCCCAGGCTTCTATAT GCTCAGATGTGGATGGCTTCCCTACAAGCCAGGCCACCAGTCCTGAGCCTGGGAAAAAGGATGAGGAGGC GAAAGCACCCTTCAAGTCCACTCTGTTCCATTTCATGGAAAAGTCCACAAATGTGGTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85656. Forward Primer - name:085656_F_cDNA_9430020K01Rik, sequence:CACAAAAGCAGCCCCTTTATAC; Reverse Primer - name:085656_N_SP6_cDNA_9430020K01Rik, sequence:CACCACATTTGTGGACTTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8047 same embryo
	EMAGE:8045 same embryo
	EMAGE:8042 same embryo
	EMAGE:8044 same embryo
	EMAGE:8046 same embryo
	EurExpress:euxassay_015963 same experiment
	MGI:4822804 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source