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EMAGE:8149

Whamm WAS protein homolog associated with actin, golgi membranes and microtubules ( MGI:2142282)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149
"Pseudo-wholemount" of euxassay_016424. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016424_01 euxassay_016424_02 euxassay_016424_03 euxassay_016424_04
EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149
euxassay_016424_05 euxassay_016424_06 euxassay_016424_07 euxassay_016424_08 euxassay_016424_09
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EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149
euxassay_016424_15 euxassay_016424_16 euxassay_016424_17 euxassay_016424_18 euxassay_016424_19
EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149 EMAGE:8149
euxassay_016424_20 euxassay_016424_21 euxassay_016424_22 euxassay_016424_23 euxassay_016424_24
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euxassay_016424_25

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8149Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8149_wholemount_strong.wlz
8149_wholemount_moderate.wlz
8149_wholemount_weak.wlz
8149_wholemount_possible.wlz
8149_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8149_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63116
Entity Detected:Whamm, WAS protein homolog associated with actin, golgi membranes and microtubules ( MGI:2142282)
Sequence:sense strand is shown

>T63116
CTGAAGAAGCAGTGGGCTCTATCCAAGACATCACTGTGAATTATTTTAAAGAAACAGTAACAGCACTAAC
AGGAATGCAGAAACAAATGGAGCAGGATCAGAAGAGATTTGGCCAGGCTGCCTGGGCCACAGCCATGCCC
AGGCTAGAAAATCTCAAGCTCATGTTAGCTCGGGAGACTCTGCAGCTTATGAGAGCCAAAGAGCTGTGTC
TAAAACACAGGCAAGCTGAAATCCAGAGAAAGGTGGAAGATCTTCCGCGCCAAGGAAAGCAGTTAGATGT
TGTGGATGAGCTGGAGATACAGTGTTATGAGATCCAGTTGGAGTTGTATGATGTCAAGCTTGAGATGCTG
AGAAATGAGGAGATAATCCTTGTCACGCGGTTGGACTCTGTTAAGAGACTTATAACAGAGAAGCAGGCTG
AAGTGATCTACTACGACCCGTGTGAGAGCCCCGAGGAGCTGCAGAGCCTGGCTCCTGACCTGGAACTGCA
CCTTGGTGACAACAGAGAGCTGAGAGCACTCAGCCAGCAGTGCCAGCGGCTGGAGGCTCAGCGGGGCCGC
ATCTGCTCCAGGAGAGCCCTCCTTAGGAACAGAAAGGATCACTGCCGAGAAAATCATCAGCTCAGACTGC
AGCAGGCCAAACAAAGTCTGAGACACCTTCACCAGCACCACAGTATTCAAATGAAGAGAGACAAAGTAAA
AGAGGAAGAACAAAAGAAAAAAGAATGGATAGACCATGAACGCCAGAAAACCCTTGAGCGGCTAAGAGCA
TATAAGGAGAAGTGCCCGGCTCATCGGTCTGCACTGAAAACAACT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 146855. Forward Primer - name:146855_F_cDNA_MGC51670, sequence:CTGAAGAAGCAGTGGGCTCTAT; Reverse Primer - name:146855_N_SP6_cDNA_MGC51670, sequence:AGTTGTTTTCAGTGCAGACCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8147 same embryo
 EMAGE:8148 same embryo
 EMAGE:8146 same embryo
 EMAGE:8151 same embryo
 EMAGE:8150 same embryo
 EurExpress:euxassay_016424 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS