EMAGE mouse gene expression spatial database

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EMAGE:8158

Dgkk diacylglycerol kinase kappa ( MGI:3580254)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016434. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016434_01	euxassay_016434_02	euxassay_016434_03	euxassay_016434_04

euxassay_016434_05	euxassay_016434_06	euxassay_016434_07	euxassay_016434_08	euxassay_016434_09

euxassay_016434_10	euxassay_016434_11	euxassay_016434_12	euxassay_016434_13	euxassay_016434_14

euxassay_016434_15	euxassay_016434_16	euxassay_016434_17	euxassay_016434_18	euxassay_016434_19

euxassay_016434_20	euxassay_016434_21	euxassay_016434_22	euxassay_016434_23	euxassay_016434_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8158_wholemount_strong.wlz

8158_wholemount_moderate.wlz

8158_wholemount_weak.wlz

8158_wholemount_possible.wlz

8158_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8158_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 11 15

pons mantle layer

weak

regional

weak expression: see section 07 17

ventral grey horn

moderate

regional

moderate expression: see section 09 14 weak expression: see section 10

lung

moderate

regional

moderate expression: see section 05 weak expression: see section 03 04 06 07 08 09 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63365
Entity Detected:	Dgkk, diacylglycerol kinase kappa ( MGI:3580254)
Sequence:	sense strand is shown >T63365 ACCTTGGGCAACTACAGTGTTTATTGGGGACAGGCTCTCTATGAATGGCCTGGGAGCTATGAGAATGTAT GGGAGTGCATCTCGTTAGGAAATATTAGAATGGGGGGTCTTTCACTTGGAGAGAAACTGTTGACACCAAG GAATAAATGAGTGACCAAGTCTCAGAAAGATGAGGAAGGGGAAGGGTGTCCAGGCTTAGTTGTGTTTCTC CTCCAATCCCTGCCAATTGCAACTTGAATTTCAAAAGATGGCTCCTAGTTGGCTTAAGAAGATGAGAACA AGGTCCTGGGGCTTGTCCACTCATCTGTTACAATGACTATGTGAATCTTTTCTTATAGATATAGAACTCT CCTCTTCTCTCTTTCCTTTCTCCTCTCATTTCCTCTTCTCTCCTCTACCACCTCTTATCTCCTCTACATC TCTTCTCTCTCCTCTCACTTCTCTCTTTCTCCACCTCCTTCTAATTTCTTTCCCCTTTCTTTCCTTTCCA TCTCTCCTTCTCTTCCACTTTCCTCTATTACTCCTTATCTCCCCATAAACTCTTCGTGTTTTTCCATGTG TACATGTATGTCTTTGCACATGTCCAGGTGGTGCTCTCGTGGCTGGGTGGGCCCACTGGTTGGAACTCAC CCCTCATGGCAACCATACAGGCTCCTGTACTTTTGCCTTGATCCCAGCCTGCATGTCTCCATGGAGTGCT TGCCTGCATTCTCGCTGGATTTTTTAACTAAATGTTTTGCCGCTGTGCTGCAGTATAGATTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105902. Forward Primer - name:105902_F_cDNA_C130007D14, sequence:ACCTTGGGCAACTACAGTGTTT; Reverse Primer - name:105902_N_SP6_cDNA_C130007D14, sequence:TAATCTATACTGCAGCACAGCGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8157 same embryo
	EMAGE:8159 same embryo
	EMAGE:8160 same embryo
	EurExpress:euxassay_016434 same experiment
	MGI:4824293 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source