EMAGE mouse gene expression spatial database

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EMAGE:8185

Spock2 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2 ( MGI:1891351)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005249. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005249_01	euxassay_005249_02	euxassay_005249_03	euxassay_005249_04

euxassay_005249_05	euxassay_005249_06	euxassay_005249_07	euxassay_005249_08	euxassay_005249_09

euxassay_005249_10	euxassay_005249_11	euxassay_005249_12	euxassay_005249_13	euxassay_005249_14

euxassay_005249_15	euxassay_005249_16	euxassay_005249_17	euxassay_005249_18	euxassay_005249_19

euxassay_005249_20	euxassay_005249_21	euxassay_005249_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8185_wholemount_strong.wlz

8185_wholemount_moderate.wlz

8185_wholemount_weak.wlz

8185_wholemount_possible.wlz

8185_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8185_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9103
Entity Detected:	Spock2, sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2 ( MGI:1891351)
Sequence:	sense strand is shown >T9103 CCTCTCCGCGTCCAGCGCTGGGCTTTTTCAGACAAGTGCATCTGCTAACCAGGTCACATTTCAGCCACGA CCCACCCTCCGTCAGTCACTGGAGTCAGACAGCAGGAGGAGGGCTGAGGAGGACCGCAGCGCTTCGCCAG TGGCGCGGTGGAGGGAAGGACATCAAATCCTGCTGAAGTCCAGACCCCCCCAGGTCCAAGCCCAGACCAC GATGCGCGCTCCAGGCTCCGGGCGGCTGGCTCTGCCGCTGCTGCTCCTCGCCGTGGTCGCCTTGGCTGAA GGCGACGCCAAGGGGCTCAAGGAGGGCGAGACCCCCGGCAATTTCATGGAGGACGAGCAATGGCTGTCGT CCATCTCGCAGTACAGCGGCAAGATTAAGCACTGGAACCGCTTCCGAGACGAGGTAGAAGATGATTACAT CAAGAGCTGGGAAGACAATCAGCAAGGAGATGAAGCTCTGGATACCACCAAGGACCCCTGCCAGAAGGTG AAGTGCAGTCGCCACAAGGTGTGTGTTGCTCAAGGCTACCAGCGCGCCATGTGCATCAGCCGCAAGAAGT TGGAGCACAGGATCAAGCAGCCTTCTCTGAAACTCCACGGAGGCAAAGACTCCGTCTGCAAGCCCTGTCA TATGGCCCAGCTCGCTTCAGTCTGTGGCTCCGACGGCCATACCTACAGCTCTGTGTGTAAGCTGGAGCAG CAGGCATGCCTAAGCAGCAAGCAGCTGGCGGTGAGATGTGAGGGCCCCTGTCCCTGTCCCACGGAGCAGT CTACTGCGTCTACCACCGACAGCAAGTCAGAGACCTGCACAGGGCAGGACCTGGCCGACCTGGGGGATAG GCTCCGTGACTGGTTCCAGCTCCTTCGTGAGAACTCCAAGCAGAACGGCTCAGCCAACAGTGCAACTAAC CCCGCAGGGTTGGACAAGAGCCTGGGGGCCAGTTGCAAGGACTCCATCGGTTGGATGTTCTCTAAGCTGG ACACTAGTGGAGACCTCTTCCTGGACCAGACAGAACTGGCTGCCATCAACCTGGACAAATACGAGGTCTG CATCCGCCCCTTCTTCAACTCCTGTGATACCTACAAAGATGGCCGCGTCTCTACTGCTGAGTGGTGCTTC TGTTTCTGGAGGGAGAAGCCCCCCTGCCTGGCAGAGCTGGAGCGCACACAGATCCAGGAGGCAGCCAAGA AGAAGCCAGGTGTCTTCATCCCGAGCTGTGATGAAGATGGCTACTACCGGAAGATGCAGTGTGACCAGAG CAGAGGTGACTGCTGGTGTGTGGACCAGCTGGGCCTGGAGCTGACCGGCACACGCATGCATGGGACCCCT GACTGTGATGACATCGTGGGTTTCTCCGGGGACTTTGGAAGCGGCGTTGGCTGGGAGGATGAGGAAGAAA AGGAGACAGAAGAGGCAGGGGAGGAGGCCGAGGAGGAGGAGGGTGAGGCGGGTGAGGCAGATGATGGAGG CTACATCTGGTAGATAGCCCTCGACGGCCTCGGCAGGCCAGGGTGCCGACGGCCAGAAAGACAGGCCAGA AGAGACCTGGACAACAGCAGGCAACTTAGCAAATGGATCCGGAAGTCAGTGCGGAGGACCCTGGCTCCAG TGGGGGAGGACTGGGGAGTGTGGCTTGTGTGTATGTGTGATATGAGATGTGTGTGTATGATGTGAGATGT GAGTGTGTGATGTGAGATGTGTGTATGTGAGATGTGTGTGTGTGTGTGTGTGTGTGTGATGTGAGTCACC CAAAGGTCCCTATGTTATTGATTTTCAAATGCACACTTTCACACACAAGGATACACTAACAGACCAAAAT CAATGAGATGTCCCTGATCCTGCCCCTCAGCCCAACACCTGGAATCCCTGCCCTGCCTAATCAGCCCTAC CCCATCCTGCCTTTCTTCCTTCCTCTTCTGAGGCCAGGGAGAGGGAGGGAGGGCAGGCAGCTGAAAGCTG AGCGTCCTCCCCAAATTGGGCTGATTTGATATGAAAGCCCCACCACGGTCTTGTGGTGATGAGGCAGGAC CTGTACACACAGCAAGGCTTACTATCCGTCTCCTGACATCCGAAGAGCTCGTCGCAGGTGTGTCTAATGG CCCTTGGGCTGCACGTGGTTAAGCATAGCTGTCTCTGTGGCCCAACACAAAGAGGTAAACTTACTTAAAA CACAGGGGTTTTGTTGCTGTTTGTTTTTTGTTAGTTTGTTTGTTGATAATTGGATTGAGGAGTTAATCCT CTGTCACAATGTCAAAAGCATGGACATCCCTGATAGATTATCTCTCAACCTAGTCCCGAGTAACCAGAGA TAGCTCAGCAGAAGAGGCAGAAATCATGAGACAGAGGACAATGGGTCCCTTGATTTCAGGCCTCAGATTA ACCCAGTCAGCACCTGTGGCCTCATGGGGCTCTGGGCTGCTTTCTTGGCGAATGGGGACTGTCATCATAT AGCTGAGATTTGGAGTAGCCTGCTATGAGGGCTTGCAGTTGCACTCTCTGTTCCCTGGCGAGGGTCCCCC TTGTCCCAAGGAGCAGAGGACCAGCAAGCAGCCCGTGTCTGCTCACCTTCTCCCTGACCCTCTAGGAAAT GGTTCCCTCTTGCCCCGACCAATAGAAGGGCGTGTCCCCGAGACCCTTGTGAGGAACCACGGGGAGCTGT GACTTCCTGTGCCCAGAAGGTCCTGGAGGAGAGGCCCCAAAGCCTTAGTCCTCTCAGCCATGACTTTCCT CTGTCTCAAGGGTCCCCAAGATGGCCCTAGAAGAACCATGCCCCAGACTCTATTTAAAGCCCTATAGCAT ATATGAATAGAGAACGGTGTATCTATCAGAGGGGACCACACTGAGCAGCTAGGCCCTGATGCCATGCCTC AGCACGCCCTCAGACCTCCAGGCCGGGTGGAAGGGGCTTTTACCCAGATGTCCTGTGGGGTTTTGTGTGT CTATTGCTCCCCACTTCCCCCTCTCAAAGGAAATTTCCTTGATATATTTGATGAAGAATTCCTTACCTGG GCCTCTGATACCCTTTGGTACGCGTACGTGTGTGTGGCGCGTATACGTGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAAGAGAGAGAGAAT GAGAGAGAATGAGAGAGAGAATACCCGAGGGCCACCCTGATGCCCAGGAAGAGTGAGGGCAGCCCCCTGT GTGGTGGAAGAGGCTGGGCAGCTAGCTACTGGGGACCTTGTGCTGAGGCTTCCAGGACAAAGCCCCAGTC TGGCTCTCCCCCAGCCTCTCAGGGGCACTGGCTGGTCACATCCACTGAGTATTGATCGGTTGGACTTTAG GGAAGAGCCTCCTCCTTCCCATTCCTTTCTGCCACCCTGGAATGGCCACCTTAATCGTCATCTCCTCGGG GGGAATACGGAAATGTCACCAAGCTCCATAAAGTGCTTCTCATTCCACGACACAGTTCTGACTCTCCGGG TGACTGTCACCTGCTCCCATCCTCTTCTTCCTCCCTCCCTTCCTCAGACCTCTGCAACCATCCTCTGGTC CCTGGCTCAGTCACATCAGCCCTCTGCATTCTAGAAGCAGCCATCCCGCCCCCCTCTCTCCTCCCCCTCC TCCCCTTCCTCCTCCCTCCAGCTGGAGTTACTTGAAACTTCCTGCTCTCGCTAGGATGGAGTCTGATTCT TTCTCTGCCCCAGCTGGATTTCCAGAACATCCCCGGGTAGCTGTGAAGGATTTTAGGCTCTCTGGGGTGG GGAGGGGGTGGTGTAGAGGAAGACGCATCCCCTTCTTTCACCCTGCCCCGCCCTGCCCCGCTGGGGAGCT ATGCTCTGTGTGTCCCTGGATCTTCTGGTTTTGTATCTGATGTAGTAAACATGTTTTAGTAAGAAAAAAA AAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6821449 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6821449 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8186 same embryo
	EurExpress:euxassay_005249 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source