EMAGE mouse gene expression spatial database

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EMAGE:8265

1300001I01Rik RIKEN cDNA 1300001I01 gene ( MGI:1921398)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007554. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007554_01	euxassay_007554_02	euxassay_007554_03	euxassay_007554_04

euxassay_007554_05	euxassay_007554_06	euxassay_007554_07	euxassay_007554_08	euxassay_007554_09

euxassay_007554_10	euxassay_007554_11	euxassay_007554_12	euxassay_007554_13	euxassay_007554_14

euxassay_007554_15	euxassay_007554_16	euxassay_007554_17	euxassay_007554_18	euxassay_007554_19

euxassay_007554_20	euxassay_007554_21	euxassay_007554_22	euxassay_007554_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8265_wholemount_strong.wlz

8265_wholemount_moderate.wlz

8265_wholemount_weak.wlz

8265_wholemount_possible.wlz

8265_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8265_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pectoral girdle and thoracic body wall musculature

moderate

regional

moderate expression: see section 03 04 05 18 19 20

adrenal gland

moderate

regional

moderate expression: see section 06 07 08 14 15 16 17

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18 19

anterior naris

moderate

regional

moderate expression: see section 13 14 15 16

external naris

moderate

regional

moderate expression: see section 16

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

kidney calyx

moderate

regional

moderate expression: see section 06 15 16 17 weak expression: see section 07 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35356
Entity Detected:	1300001I01Rik, RIKEN cDNA 1300001I01 gene ( MGI:1921398)
Sequence:	sense strand is shown >T35356 GCTCTGAGTACCTCAAGTGCCTGACCCAGCAGGCTGTGGCCCTGCAGCGCACCATGAATGAGATCTACCG TAATGGCTCCAGTGCCAATATCCCACCCCTCAAGTTCACAGCTCCCAGCATGACCAGTGTCCTGGAGCAG CTCAATGTCATCAATGGCATCCTCTTCATTCCTCTCAGCCAAAAAGACTTGGAAAACCTGAAGGCAGAGG TGGCACGGCGACACCAGCTCCAAGAGGCCAACAGAAACAGGGATAAGGCTGAAGAACAACCCATGGCCCC CGAGCCTGAGCCTGAGCCAGAGAGAGCCGTGGAGGACATGGGCTCCCCTCAGACTGCCAAGGAAGGTCCT TCTTCCCTGAACTTACAGGGATAAAAAAAGAAAGAAAGATGGACAGTCCAGCAGCCCCATTACCCGGCGA TGCAGACAGATTCCAGGAGAATTCTGGGGGCGTGCCCTGCAGATTGGGAGAGGAAGCTAGTCCTCAACTT TATACCCCCACCTCACCCCACCATCTGCCTGGGGCCCTGCCCTGGTTTGCCTGCCCCTAAAAGATGGTTT TGCACTGGTTCAATGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87546. Forward Primer - name:087546_F_cDNA_1300001I01Rik, sequence:GCTCTGAGTACCTCAAGTGCCT; Reverse Primer - name:087546_N_SP6_cDNA_1300001I01Rik, sequence:TCATTGAACCAGTGCAAAACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8266 same embryo
	EMAGE:8267 same embryo
	EurExpress:euxassay_007554 same experiment
	MGI:4822604 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source