EMAGE mouse gene expression spatial database

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EMAGE:8278

AI464131 expressed sequence AI464131 ( MGI:2140300)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007615. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007615_01	euxassay_007615_02	euxassay_007615_03	euxassay_007615_04

euxassay_007615_05	euxassay_007615_06	euxassay_007615_07	euxassay_007615_08	euxassay_007615_09

euxassay_007615_10	euxassay_007615_11	euxassay_007615_12	euxassay_007615_13	euxassay_007615_14

euxassay_007615_15	euxassay_007615_16	euxassay_007615_17	euxassay_007615_18	euxassay_007615_19

euxassay_007615_20	euxassay_007615_21	euxassay_007615_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8278_wholemount_strong.wlz

8278_wholemount_moderate.wlz

8278_wholemount_weak.wlz

8278_wholemount_possible.wlz

8278_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8278_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 13 weak expression: see section 12

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 13 weak expression: see section 12

telencephalon ventricular layer

moderate

regional

moderate expression: see section 11 16 17 weak expression: see section 08 09 10 14 15

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 09 10 13 14 weak expression: see section 11 12

pons ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 14 15 16 17 weak expression: see section 11 12 13 18

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 14 15 weak expression: see section 11 12 13

spinal cord ventricular layer

moderate

regional

moderate expression: see section 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35901
Entity Detected:	AI464131, expressed sequence AI464131 ( MGI:2140300)
Sequence:	sense strand is shown >T35901 CAAACCCAGTTTAAGAGTTCCGAAACTGGAGAGTCGGGCTGAGGAACTGAGTTAGGTTGAACTTGGGAAA GCAAGCCCAAGTTCAACCTAACTCCTGCTGTAAAATGGAGAGAACTGTAGCAAATGAACAGCCCTTTAAG ATTACCTAGCTGCCTCTCTGAACACACACACACACACACACACACACACACACGCACGCACGCACGCGCG TGCTCTGAGTGAAGCCTTAGTGGGAAAACCATGTTCTAATTCTGGCCGAGATGTGCTTGGCACATGAATG ACATTACACACTGATGCCTGCCAACACACGCATGGGGTAAGGGCTTTCATCTCAGTAATTCTCTTGTTCT AAGGAGATCAGCTCCAACTATGGTGGGGTGAGTTTTTCCTGACTTGGAGGCTTGCCAGAGAAGGTGGAGA ATGAAGTAGAACTCAGCTTCCCTCTCAGAGCCTGTGCCCGAATCTTTAATGTTCTCTGGGTGAAAGGTCC TGGGGAGAGCTTCGGGGCTCCCCCGATGCCCATCTCCAATGCCAATTCTTTGGTTTCTTCTTCTTGGCAG TGTTGAAGCTAGAACTCAGGGCGCTGAATACTAAGCAAGCATTCTTCCGGGAGCCATATTCGCAGTCTTC GTTTGTCAGTCAGCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77827. Forward Primer - name:077827_F_cDNA_AI464131, sequence:CAAACCCAGTTTAAGAGTTCCG; Reverse Primer - name:077827_N_SP6_cDNA_AI464131, sequence:CCAGCTGACTGACAAACGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8280 same embryo
	EMAGE:8279 same embryo
	EMAGE:8277 same embryo
	EMAGE:8281 same embryo
	EurExpress:euxassay_007615 same experiment
	MGI:4823045 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source