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EMAGE:8284

Atp5g3 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9) ( MGI:2442035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284
"Pseudo-wholemount" of euxassay_007728. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007728_01 euxassay_007728_02 euxassay_007728_03 euxassay_007728_04
EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284
euxassay_007728_05 euxassay_007728_06 euxassay_007728_07 euxassay_007728_08 euxassay_007728_09
EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284
euxassay_007728_10 euxassay_007728_11 euxassay_007728_12 euxassay_007728_13 euxassay_007728_14
EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284
euxassay_007728_15 euxassay_007728_16 euxassay_007728_17 euxassay_007728_18 euxassay_007728_19
EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284 EMAGE:8284
euxassay_007728_20 euxassay_007728_21 euxassay_007728_22 euxassay_007728_23 euxassay_007728_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8284Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8284_wholemount_strong.wlz
8284_wholemount_moderate.wlz
8284_wholemount_weak.wlz
8284_wholemount_possible.wlz
8284_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8284_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35960
Entity Detected:Atp5g3, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9) ( MGI:2442035)
Sequence:sense strand is shown

>T35960
TTCTGCATCAGTGTTATCTCGGCCAGAGACTAGGACTGGAGAGGGCTCTACAGTTTTTAATGGGGCCCAG
AATGGTGTGTGTCAGCTGATCCGAAGGGAGTTTCAGACCAGTGTAATCAGCAGAGACATTGATACTGCTG
CCAAATTCATTGGTGCAGGTGCTGCAACAGTAGGAGTTGCTGGTTCTGGTGCTGGTATTGGAACAGTCTT
TGGCAGTCTTATCATTGGTTATGCCAGAAACCCTTCACTGAAGCAGCAGCTGTTCTCATATGCTATCCTG
GGATTTGCCTTGTCTGAAGCTATGGGTCTCTTTTGTTTGATGGTTGCGTTCTTGATCTTGTTTGCCA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 93514. Forward Primer - name:093514_F_cDNA_Atp5g3, sequence:TTCTGCATCAGTGTTATCTCGG; Reverse Primer - name:093514_N_SP6_cDNA_Atp5g3, sequence:TGGCAAACAAGATCAAGAACG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8285 same embryo
 EMAGE:8283 same embryo
 EMAGE:8286 same embryo
 EMAGE:8282 same embryo
 EurExpress:euxassay_007728 same experiment
 MGI:4823313 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS