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EMAGE:8455

Rasgef1c RasGEF domain family, member 1C ( MGI:1921813)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455
"Pseudo-wholemount" of euxassay_009648. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009648_01 euxassay_009648_02 euxassay_009648_03 euxassay_009648_04
EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455
euxassay_009648_05 euxassay_009648_06 euxassay_009648_07 euxassay_009648_08 euxassay_009648_09
EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455
euxassay_009648_10 euxassay_009648_11 euxassay_009648_12 euxassay_009648_13 euxassay_009648_14
EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455
euxassay_009648_15 euxassay_009648_16 euxassay_009648_17 euxassay_009648_18 euxassay_009648_19
EMAGE:8455 EMAGE:8455 EMAGE:8455 EMAGE:8455
euxassay_009648_20 euxassay_009648_21 euxassay_009648_22 euxassay_009648_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8455Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8455_wholemount_strong.wlz
8455_wholemount_moderate.wlz
8455_wholemount_weak.wlz
8455_wholemount_possible.wlz
8455_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8455_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
olfactory cortex marginal layer
weak weak
regionalweak expression: see section 10 11 14 15 16
rest of cerebellum marginal layer
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 16 17 18 19
ventral grey horn
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36946
Entity Detected:Rasgef1c, RasGEF domain family, member 1C ( MGI:1921813)
Sequence:sense strand is shown

>T36946
TACCTGGTAGCAACGGAAATCTGCATGCCAGCCAAGAAGAAGCAGAGAGCGCAGGTGATCGAGTTCTTCA
TCGATGTGGCCCGAGAGTGCTTCAACATCGGCAACTTCAACTCCCTCATGGCCATTATCTCTGGCATGAA
CATGAGCCCTGTCTCCAGGCTGAAGAAGACTTGGGCCAAAGTGAAAACAGCCAAATTCTTCATTCTGGAG
CACCAGATGGACCCAACAGGGAACTTCTGCAACTATAGGACAGCCCTGCGTGGGGCAGCCCACCGCTCAC
TCACTGCTCACAGCAGCAGGGAGAAGATCGTCATCCCCTTCTTCAGTCTGCTCATCAAGGACATTTACTT
CCTCAATGAGGGCTGTGCCAACCGCCTTCCCAATGGGCATGTCAACTTTGAGAAATTTCTGGAACTGGCC
AAGCAGGTTGGAGAATTCATTACTTGGAAACAAGTGGAATGTCCTTTCGAGCAAGATCCGAGCATCACCC
ATTACCTGTACACTGCCCCCATCTTCAGCGAAGACGGTTTGTATTTGGCTTCCTATGAAAGCGAGAGCCC
AGAGAGCCAGACAGAAAAGGAGAGGTGGAAATCTCTAAGATCTTCTATTCTGGGGAAGACATGAGAAGCC
CTGACACAGAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83536. Forward Primer - name:083536_F_cDNA_Rasgef1c, sequence:TACCTGGTAGCAACGGAAATCT; Reverse Primer - name:083536_N_SP6_cDNA_Rasgef1c, sequence:CTCTGTGTCAGGGCTTCTCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8454 same embryo
 EMAGE:8451 same embryo
 EMAGE:8452 same embryo
 EMAGE:8453 same embryo
 EMAGE:8450 same embryo
 EurExpress:euxassay_009648 same experiment
 MGI:4827627 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS