EMAGE mouse gene expression spatial database

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EMAGE:8464

Rev3l REV3-like, catalytic subunit of DNA polymerase zeta RAD54 like (S. cerevisiae) ( MGI:1337131)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009657. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009657_01	euxassay_009657_02	euxassay_009657_03	euxassay_009657_04

euxassay_009657_05	euxassay_009657_06	euxassay_009657_07	euxassay_009657_08	euxassay_009657_09

euxassay_009657_10	euxassay_009657_11	euxassay_009657_12	euxassay_009657_13	euxassay_009657_14

euxassay_009657_15	euxassay_009657_16	euxassay_009657_17	euxassay_009657_18	euxassay_009657_19

euxassay_009657_20	euxassay_009657_21	euxassay_009657_22	euxassay_009657_23	euxassay_009657_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8464_wholemount_strong.wlz

8464_wholemount_moderate.wlz

8464_wholemount_weak.wlz

8464_wholemount_possible.wlz

8464_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8464_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 04 05 06 07 08

spinal cord

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 weak expression: see section 08 not examined expression: see section 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 18

vomeronasal organ

moderate

regional

moderate expression: see section 12 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36955
Entity Detected:	Rev3l, REV3-like, catalytic subunit of DNA polymerase zeta RAD54 like (S. cerevisiae) ( MGI:1337131)
Sequence:	sense strand is shown >T36955 TGGATCAGAAGGAGCCAGTATTTGATGCGAAAGGAATAGAAACAGTCAGAAGAGACTCTTGCCCTGCTGT CTCTAAGATACTTGAGCGTTCTCTAAAGCTGCTGTTCGAAACAAGAGATATTAGTCTAATTAAACAGTAT GTACATCGACAATGTATGAAACTTGTGGAAGGGAAGGCCAGCATCCAAGATTTTATCTTTGCTAAAGAGT ACAGAGGAAGTTTTTCTTACAGGCCTGGAGCCTGTGTTCCAGCCCTTGAACTTACAAGGAAAATGCTTGC TTATGATCGGCGCTCTGAGCCTCGAGTTGGAGAACGAGTGCCATATGTCATCATTTATGGGACCCCTGGA CTACCCCTTATCCAGTTGATAAGGCGCCCAGCAGAAGTCTTACAGGATCCCACTCTGAGGCTGAATGCCA CTTACTATATCACCAAACAAATTCTTCCACCCCTGGCCAGAATCTTCTCTCTTATTGGTATTGATGTCTT CAGCTGGTATCAAGAATTACCAAGGATCCAGAAAGCTACCAGCTCCTCCAGAAGTGAACTTGAAGGGCGG AAAGGCACTATCTCTCAGTATTTCACTACTTTGCACTGTCCTGTGTGTGATGACCTGACTCAGCATGGTA TCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 64190. Forward Primer - name:064190_F_cDNA_Rev3l, sequence:TGGATCAGAAGGAGCCAGTATT; Reverse Primer - name:064190_N_SP6_cDNA_Rev3l, sequence:AGATACCATGCTGAGTCAGGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8466 same embryo
	EMAGE:8462 same embryo
	EMAGE:8463 same embryo
	EMAGE:8465 same embryo
	EMAGE:8461 same embryo
	EurExpress:euxassay_009657 same experiment
	MGI:4827701 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source