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EMAGE:8474

Rhag Rhesus blood group-associated A glycoprotein ( MGI:1202713)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474
"Pseudo-wholemount" of euxassay_009664. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009664_01 euxassay_009664_02 euxassay_009664_03 euxassay_009664_04
EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474
euxassay_009664_05 euxassay_009664_06 euxassay_009664_07 euxassay_009664_08 euxassay_009664_09
EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474
euxassay_009664_10 euxassay_009664_11 euxassay_009664_12 euxassay_009664_13 euxassay_009664_14
EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474
euxassay_009664_15 euxassay_009664_16 euxassay_009664_17 euxassay_009664_18 euxassay_009664_19
EMAGE:8474 EMAGE:8474 EMAGE:8474 EMAGE:8474
euxassay_009664_20 euxassay_009664_21 euxassay_009664_22 euxassay_009664_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8474Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8474_wholemount_strong.wlz
8474_wholemount_moderate.wlz
8474_wholemount_weak.wlz
8474_wholemount_possible.wlz
8474_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8474_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
liver lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36962
Entity Detected:Rhag, Rhesus blood group-associated A glycoprotein ( MGI:1202713)
Sequence:sense strand is shown

>T36962
GACTCAGATGTGAACACCCAAATGATGAATCTGTGTACCACTCTGACTTGTTTGCCATGATCGGAACACT
TTTCCTGTGGATATTTTGGCCCAGCTTTAATTCAGCCATTGCTGATCCTGGAGATCATCAGTATAGGGCC
ATTGTCAACACATACATGTCCCTTGCAGCCTGTGTGATCACAGCCTATGCCTTGTCCAGCCTTGTAGAGC
GCCGAGGCAGGCTGGATATGGTACACATTCAGAATGCTACTCTAGCAGGAGGTGTTGCTGTGGGCACATG
TGCAGACATGGAAATCCCCCTATATGCTGCTATGACCATTGGAAGCATTGCAGGGATCATCTCTGTGCTT
GGATACAAGTTCTTTAGTCCACTGTTAGCTAATAAACTGATGATCCATGATACATGTGGGGTTCATAACT
TGCATGGCTTACCTGGAGTTTTTGGAGGCCTTGCCAGCATTGTGGCCATAAGCTGGGGGATGTCTACTGC
GTCTATGGCTATGCAGGCAGCAGCACTGGGATCTTCCATTGGCTCAGCGATTGTTGGAGGTTTGCTTACA
GGTCTAATTCTGAAGTTGCCTATCTGGAACCAGCCACCTGATGAATACTGCTATGATGACTCTGTTTCTT
GGAAGGTTCCCAAATTCAGAGAACTTGATAATCGCTTCTTTCAACATGCAAATCACAACCACGTGGAACA
TGAAGTCTAAAAGCTGATCCTAAACTCTGCATCCTCGGAATTGCCAAGAAGAGTCAAATTTAAAGAAACA
AAAACTTGGTATCCAAAGAGATAGGCATGACAATAGATAAATTCCAGCCCCAAATGGCCAGTGTACAAAT
GTGTTTAGTTTTACTATTATTCCATGCTGTCCAAATGATTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90341. Forward Primer - name:090341_F_cDNA_Rhag, sequence:GACTCAGATGTGAACACCCAAA; Reverse Primer - name:090341_N_SP6_cDNA_Rhag, sequence:GAAATCATTTGGACAGCATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8478 same embryo
 EMAGE:8476 same embryo
 EMAGE:8477 same embryo
 EMAGE:8473 same embryo
 EMAGE:8475 same embryo
 EurExpress:euxassay_009664 same experiment
 MGI:4827725 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS