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EMAGE:8510

Gpr123 G protein-coupled receptor 123 ( MGI:1277167)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8510 EMAGE:8510 EMAGE:8510 EMAGE:8510 EMAGE:8510
"Pseudo-wholemount" of euxassay_009756. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009756_01 euxassay_009756_02 euxassay_009756_03 euxassay_009756_04
EMAGE:8510 EMAGE:8510 EMAGE:8510 EMAGE:8510 EMAGE:8510
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euxassay_009756_15 euxassay_009756_16 euxassay_009756_17 euxassay_009756_18 euxassay_009756_19
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euxassay_009756_20 euxassay_009756_21 euxassay_009756_22 euxassay_009756_23 euxassay_009756_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8510Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8510_wholemount_strong.wlz
8510_wholemount_moderate.wlz
8510_wholemount_weak.wlz
8510_wholemount_possible.wlz
8510_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8510_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata basal plate mantle layer
moderate moderate
single cellmoderate expression: see section 10 11 14
spinal cord floor plate
moderate moderate
regionalmoderate expression: see section 10 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30611
Entity Detected:Gpr123, G protein-coupled receptor 123 ( MGI:1277167)
Sequence:sense strand is shown

>T30611
TTCTGCGTGACACTGGGACTCTTTGTACTTATCCATCACTGCGCCAAGCGGGAGGACGTGTGGCAGTGCT
GGTGGTCCTGCTGCCCTTCCCGGGGAGACACCTCCACCACCAAGCCCGGCGCCCATCCTACACTCGATGC
CAACGGGGATGCGCTGGGGCATACGGCCTGCCTGCAGGACTCACCATGCCCTGGGAAACTCAGAGGTTTT
GGCCATCCACCAGCCAGCCACTGCAAGATGACCAACCTGCAGGCTGCCCAGGGCCATGTCAGCTGTCTGT
CACCTGCCACCCCGTGCTGTGCCAAGATGCACTGTGAGCAGTTAATGGAGGAGGAGGCGGCCCACATCCA
CATGGCTGAGGAGGACGTCTACCCACACGATCCCCACCTGCATGACCCTCACCTGCACAGGTGCCTCAAG
GGCAGAACTAAGTCCCACTACTTCAGCCGGCACCAGGCAGCTGCGGCTGAGAGGGAGTATGCTTACCACA
TCCCCTCCAGCCTGGATGGCAGTCCCCACAGTTCACGTTCGGAGAGCCCCACCAGCTCTCTGGAGGGCCC
AATGGGGATGCACACATTGGCCTGCTGTGCCCAGGCTGACCCCTTCCCCATGGTCAGCCAGCCTGAGGGT
GGAGACACAAGCCCTGGGCTCTATGGCTGCCCCCCCACCTATCCCCAGGCCCTGCCCACTTGGAGATGCT
ACGAAGGACACAGTCCCTGCCTTTTGGGGGCCCCAGCCAGAATGGGCTGCTCCAGGGTGATGTTCGAGAA
GGCCTGCCATTTGGCACTGATGGGACAGGGAACATCCGAACAGGACCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5704932), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59913. Forward Primer - name:059913_F_IRAV114_h12_Gpr123, sequence:TTCTGCGTGACACTGGGA; Reverse Primer - name:059913_R_SP6_IRAV114_h12_Gpr123, sequence:CAGGGTCCTGTTCGGATG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8513 same embryo
 EMAGE:8512 same embryo
 EMAGE:8509 same embryo
 EMAGE:8511 same embryo
 EMAGE:8508 same embryo
 EurExpress:euxassay_009756 same experiment
 MGI:4825170 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS