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EMAGE:8563

Far2 fatty acyl CoA reductase 2 ( MGI:2687035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563
"Pseudo-wholemount" of euxassay_009775. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009775_01 euxassay_009775_02 euxassay_009775_03 euxassay_009775_04
EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563
euxassay_009775_05 euxassay_009775_06 euxassay_009775_07 euxassay_009775_08 euxassay_009775_09
EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563
euxassay_009775_10 euxassay_009775_11 euxassay_009775_12 euxassay_009775_13 euxassay_009775_14
EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563
euxassay_009775_15 euxassay_009775_16 euxassay_009775_17 euxassay_009775_18 euxassay_009775_19
EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563 EMAGE:8563
euxassay_009775_20 euxassay_009775_21 euxassay_009775_22 euxassay_009775_23 euxassay_009775_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8563Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8563_wholemount_strong.wlz
8563_wholemount_moderate.wlz
8563_wholemount_weak.wlz
8563_wholemount_possible.wlz
8563_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8563_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
anterior naris
strong strong
regionalstrong expression: see section 13 17
lower jaw alveolar sulcus
strong strong
regionalstrong expression: see section 09 10 moderate expression: see section 08 11 12 16 18 19 20
upper jaw alveolar sulcus
strong strong
regionalstrong expression: see section 09 10 moderate expression: see section 08 11 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30640
Entity Detected:Far2, fatty acyl CoA reductase 2 ( MGI:2687035)
Sequence:sense strand is shown

>T30640
GCAGACCTCAACCAGCGTGACTTCGCCATCAGCAAGGAAGACGTGCAAGAACTTCTGTCCTGTACCAATA
TTATCTTCCACTGTGCAGCCACAGTGCGCTTTGATGCACATCTGAGAGAAGCTGTGCAGCTGAACGTCAC
GGCCACCCAGCAGCTCCTGCTAATGGCCAGCCAGATGCCAAAGCTGGAAGCCTTCATCCACATCTCCACT
GCCTTTTCCAACTGCAACCTGAGCCACATTGATGAGGTCATCTACCCATGCCCTGTGGAGCCAAGAAAGA
TCATTGACTCCATGGAGTGGTTAGATGACTCTATCATTGAAGAGATCACTCCTAAGCTGATTGGGGACCG
GCCCAATACCTACACCTACACCAAAGCCTTGGGAGAGATAGTAGTGCAGCAAGAGAGCGGAAACCTGAAT
GTGGCCATTGTCAGGCCATCCATAGTGGGAGCAACCTGGCAGGAGCCTTTCCCGGGTTGGGTTGATAATC
TAAATGGACCCAGCGGACTCATTATTGCGACTGGGAAAGGATTTCTTCGGTCCATAAAAGCGACTCCCAT
GGCAGTGGCAGATGTGATTCCAGTTGACACAGTTGTTAATCTCACCATAGCAGTAGGATGGTATACTGCA
GTTCATAGGCCTAAATCAACATTAATCTACCATTCCACATCTGGTAATCTCAATCCCTGTAACTGGTATA
AAATGGGATTACAGGTCTTGGCGACCATCGAAAAGATCCCATTTGAGAGTGCTTTCAGAAGGCCAAATGC
TGACTTCACCACCAGCAATTTCACCACCCATTATTGGAACACCGTCAGCCACCGGGTCCCAGCCATCATC
TATGACTTCTATCTGAGGCTTACTGGAAGGAAACCCAGAATGCTGAAGCTCATGAACCGGCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6809131), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59800. Forward Primer - name:059800_F_IRAV116_a09_Mlstd1, sequence:GCAGACCTCAACCAGCGT; Reverse Primer - name:059800_R_SP6_IRAV116_a09_Mlstd1, sequence:CAGCCGGTTCATGAGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8559 same embryo
 EMAGE:8564 same embryo
 EMAGE:8561 same embryo
 EMAGE:8562 same embryo
 EMAGE:8560 same embryo
 EurExpress:euxassay_009775 same experiment
 MGI:4824778 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS