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EMAGE:8630

Hck hemopoietic cell kinase ( MGI:96052)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630
"Pseudo-wholemount" of euxassay_009826. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009826_01 euxassay_009826_02 euxassay_009826_03 euxassay_009826_04
EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630
euxassay_009826_05 euxassay_009826_06 euxassay_009826_07 euxassay_009826_08 euxassay_009826_09
EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630
euxassay_009826_10 euxassay_009826_11 euxassay_009826_12 euxassay_009826_13 euxassay_009826_14
EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630
euxassay_009826_15 euxassay_009826_16 euxassay_009826_17 euxassay_009826_18 euxassay_009826_19
EMAGE:8630 EMAGE:8630 EMAGE:8630 EMAGE:8630
euxassay_009826_20 euxassay_009826_21 euxassay_009826_22 euxassay_009826_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8630Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8630_wholemount_strong.wlz
8630_wholemount_moderate.wlz
8630_wholemount_weak.wlz
8630_wholemount_possible.wlz
8630_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8630_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 19 20 21 22 moderate expression: see section 07 08 09 13 14 15 16 17 18
humerus
moderate moderate
regionalmoderate expression: see section 01 02 23
hip
moderate moderate
regionalmoderate expression: see section 05 06 07 13 weak expression: see section 14
fibula
moderate moderate
regionalmoderate expression: see section 22
tibia
moderate moderate
regionalmoderate expression: see section 22 23
femur
weak weak
regionalweak expression: see section 02 18 19 20 21
vibrissa
weak weak
regionalweak expression: see section 03 04 05 17 18 19
mandible
strong strong
regionalstrong expression: see section 02 03 21 22 weak expression: see section 04 05 06 07 15 16 20
maxilla
weak weak
regionalweak expression: see section 06 07 15 16
basioccipital bone
weak weak
regionalweak expression: see section 10 11 12 13 14 18
basisphenoid bone
weak weak
regionalweak expression: see section 10 11 12 13 14
clavicle
strong strong
regionalstrong expression: see section 16 weak expression: see section 08 09 15 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30879
Entity Detected:Hck, hemopoietic cell kinase ( MGI:96052)
Sequence:sense strand is shown

>T30879
GTGGGAGGGGACCAAAGTCGCCCGTGAAGGGGGCTCTGACCCGTTCCGAGCGCCAACGCAGCCTCTGTAG
CCCGCAAGTCTTCGTCGCTTGCTCCGGGCTCTGAAGTCCGGGGCCACCAGGGGCCGCAGCGCTGGGGGGT
CGGTCTAGCTGCGAGGATCCGGGCTGCCCACGAAGCGAAGGGCGGGCGCCCAGGATGGGATGCGTGAAGT
CCAGGTTCCTCCGAGATGGAAGCAAGGCCTCAAAAACAGAGCCAAGTGCCAATCAGAAGGGCCCTGTGTA
TGTGCCGGATCCCACGTCCTCCAGCAAGCTGGGACCAAACAACAGCAACAGCATGCCCCCAGGGTTTGTG
GAGGGCTCTGAGGATACCATTGTGGTCGCACTGTACGACTATGAGGCTATTCACCGTGAAGACCTCAGCT
TCCAGAAGGGAGACCAGATGGTGGTTCTGGAGGAGGCTGGGGAGTGGTGGAAGGCACGGTCCCTGGCTAC
CAAGAAGGAAGGCTACATCCCAAGCAACTATGTGGCTCGAGTTAACTCTTTGGAGACAGAAGAGTGGTTC
TTCAAGGGGATCAGCCGGAAGGATGCAGAGCGCCACCTCCTGGCTCCAGGCAACATGCTGGGCTCCTTCA
TGATCCGGGACAGTGAGACCACCAAAGGGAGCTACTCGTTGTCTGTTCGAGACTTTGACCCCCAGCACGG
AGACACCGTGAAGCACTATAAGATCCGGACGCTGGACAGTGGAGGCTTCTACATCTCTCCAAGGAGCACC
TTCAGCAGCCTGCAGGAACTCGTGCTCCACTACAAGAAGGGGAAGGATGGGCTCTGCCAGAAGCTGTCAG
TGCCCTGTGTGTCTCCCAAACCCCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3978518), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 18070. Forward Primer - name:018070_F_IRAV24-27_G24_Hck, sequence:GTGGGAGGGGACCAAAGT; Reverse Primer - name:018070_R_SP6_IRAV24-27_G24_Hck, sequence:TCTGGGGTTTGGGAGACA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8631 same embryo
 EMAGE:8633 same embryo
 EMAGE:8634 same embryo
 EMAGE:8632 same embryo
 EMAGE:8635 same embryo
 EurExpress:euxassay_009826 same experiment
 MGI:4825333 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS