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EMAGE:8702

Paox polyamine oxidase (exo-N4-amino) ( MGI:1916983)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8702
euxassay_009923_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8702Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8702_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8702_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31076
Entity Detected:Paox, polyamine oxidase (exo-N4-amino) ( MGI:1916983)
Sequence:sense strand is shown

>T31076
CAGCAGGACAGGAAGGGAAGGCAGAGAAGCCTTGAACTCTCATCTGGACAGGGCTTTACAAGAAATGGAA
GGTTTTCTTAAAGAACACCAAGATACCTTTTTTGAGCCACCACTGCCTGCCAAAAAAGCAGAAGCCATCA
AAAAACTAGGCTTTGGTACCAACAACAAAATCTTCTTGGAGTTTGAAGAGCCCTTCTGGGAGCCCGACTG
CCAGTTCATCCAGGTGGTGTGGGAAGATACATCGCCCTTACAGGACACAGCCCTCTCACTCCAAGACACC
TGGTTCAAGAAGCTCATTGGATTTTTGGTCCAGCCTTCTTTTGAGTCTTCACATGTGCTCTGTGGGTTTA
TTGCTGGGCTGGAGTCGGAGTTTATGGAGACTCTGTCAGATGAAGAAGTGCTTCTGTCTCTAACCCAAGT
ACTCCGGAGAGTGACAGGAAACCCACAATTGCCAGCAGCCAAGAGCGTACGAAGGTCTCAGTGGCACAGT
GCCCCGTACACCCGAGGTTCCTACAGCTATGTGGCTGTGGGCAGCACTGGCGATGACCTAGATCTGATGG
CTCAGCCCCTCCCTGAAGATGGAACTGGCACCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:1067414), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63734. Forward Primer - name:063734_F_IRAV40_g02_Paox, sequence:CAGCAGGACAGGAAGGGA; Reverse Primer - name:063734_R_SP6_IRAV40_g02_Paox, sequence:TGGGTGCCAGTTCCATCT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8699 same embryo
 EMAGE:8698 same embryo
 EMAGE:8697 same embryo
 EMAGE:8701 same embryo
 EMAGE:8700 same embryo
 EurExpress:euxassay_009923 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS