EMAGE mouse gene expression spatial database

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EMAGE:8799

Myo5b myosin VB ( MGI:106598)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010054. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010054_01	euxassay_010054_02	euxassay_010054_03	euxassay_010054_04

euxassay_010054_05	euxassay_010054_06	euxassay_010054_07	euxassay_010054_08	euxassay_010054_09

euxassay_010054_10	euxassay_010054_11	euxassay_010054_12	euxassay_010054_13	euxassay_010054_14

euxassay_010054_15	euxassay_010054_16	euxassay_010054_17	euxassay_010054_18	euxassay_010054_19

euxassay_010054_20	euxassay_010054_21	euxassay_010054_22	euxassay_010054_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

8799_wholemount_strong.wlz

8799_wholemount_moderate.wlz

8799_wholemount_weak.wlz

8799_wholemount_possible.wlz

8799_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:8799_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 13 14 weak expression: see section 10 15

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 13 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 08 09 10 11 12 13 15 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 05 06 07 16 17 18

tongue muscle

weak

regional

weak expression: see section 13 14

midgut

weak

regional

weak expression: see section 10 11 12 13 14 15 16

renal cortex

weak

regional

weak expression: see section 05 06 07 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31779
Entity Detected:	Myo5b, myosin VB ( MGI:106598)
Sequence:	sense strand is shown >T31779 CCTCCTCATCCGGAACCTGGTGACAGACCTGAAGCCTCAGATGCTGTCGGGCACCGTGCCCTGTCTGCCT GCATACATACTCTATATGTGCATCAGGCACGCGGATTACACCAACGATGACCTCAAGGTGCACTCGTTGC TGAGCTCCACCATCAACGGCATTAAGAAAGTCCTCAAGAAGCACAATGATGACTTTGAGATGACGTCATT CTGGCTATCCAACACCTGCCGCTTCCTTCACTGTCTGAAGCAATACAGTGGTGATGAGGGTTTCATGACA CAGAACACTGCGAAGCAGAATGAGCACTGTCTCAAGAACTTTGACCTCACTGAATACCGCCAGGTGCTAA GCGACCTTTCCATTCAGATCTATCAGCAGCTCATTAAAATCGCCGAGGGCTTGCTGCAGCCTATGATAGT TTCTGCCATGTTGGAAAATGAGAGTATCCAGGGGCTGTCTGGTGTGAGACCAACTGGTTACCGGAAGCGC TCCTCCAGCATGGTGGATGGAGAGAATTCGTATTGCCTGGAGGCCATCGTCCGCCAGATGAATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5060955), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 63029. Forward Primer - name:063029_F_IRAV97_d02_Myo5b, sequence:CCTCCTCATCCGGAACCT; Reverse Primer - name:063029_R_SP6_IRAV97_d02_Myo5b, sequence:GAATTCATCTGGCGGACG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:8800 same embryo
	EMAGE:8798 same embryo
	EMAGE:8801 same embryo
	EurExpress:euxassay_010054 same experiment
	MGI:4826565 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source