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EMAGE:8838

Rftn2 raftlin family member 2 ( MGI:1921263)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838
"Pseudo-wholemount" of euxassay_012996. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012996_01 euxassay_012996_02 euxassay_012996_03 euxassay_012996_04
EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838
euxassay_012996_05 euxassay_012996_06 euxassay_012996_07 euxassay_012996_08 euxassay_012996_10
EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838
euxassay_012996_11 euxassay_012996_12 euxassay_012996_13 euxassay_012996_14 euxassay_012996_15
EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838 EMAGE:8838
euxassay_012996_16 euxassay_012996_17 euxassay_012996_18 euxassay_012996_19 euxassay_012996_20
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euxassay_012996_21 euxassay_012996_22 euxassay_012996_23 euxassay_012996_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8838Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8838_wholemount_strong.wlz
8838_wholemount_moderate.wlz
8838_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8838_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38691
Entity Detected:Rftn2, raftlin family member 2 ( MGI:1921263)
Sequence:sense strand is shown

>T38691
GGGTGAAGACAGAAACAAGGTCAGCAGCAGGAGCCTAGGCCTGGACACAAATGCATCCCAAGCCGCAGGA
GGCCGAGCCCCTCTTGAGGAGGGCTCCCTCTCTCCGTCCAGGGAGTGCTGGACCAAGGAAGAGCGGCCCG
CACAGAGCGACAGCTTCTCTGGGTTCAGTAGCAGTGACAGTGTCCTCCGGGAACTCGATGACGGACAGTT
TGACCAGGAAGAGGGCGTGACACAGGTCACTTGCATGTGAGTTGCCAGGTGACCCCATCAGAGGCCTTGT
AAATAGTAATTAAATGACTGCCATCTGACTTTATTTTGCTACTACTATCTTCATTTATGTAATGTTTTTA
ATTTCCAGGCTCAGCCTTCCCCTAATCTATGTAAACTTTGGCTTAACCTCTGAGACAAGGAAGAATAACC
AGCTATTTATCAGTTTAAAAGCATTCTTACATTTCACTCAACTAATACATCTGTATAAACAGATGTCTCC
TCTGGTCATTAAGCAACAACCTGGCTCACTTCCCCACAAAGGGACAAATATCAGTATTTCTCATCAGGCC
TATTGAAAAGGCCGACTGTCCAAATCATTTCAACCAATTAGCGCCTTCTCAGGAAGCTACAGCCTCGATT
AAACTGAGTAATATTCTTTCCTCCCTATCTCTGTCCCTCTCTCCCATGGACACAGACTCATGAAAATATC
CATTCCCTGCCTATAGCAGTTAATCTAATTTTTGTCTCTTACTGTTGTATGTCCATACAAAGCAAGCCCC
ATTTGTCAAAAGTCTTTAAAAAAAATTTTTTTTTTCTTTCCTATGTCTTTTCTTTCAATAGGCCACCAGG
TGGCCAGTCCTTCAGTAAACCATTAACTTTGGCATGATTGGTCCCGATGGCCTGCCTACCAGTGGCGTTA
AACAGACTCTCTTACGTCAGTTAAGGCTGACAGGTAAAGGCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 163522. Forward Primer - name:163522_F_cDNA_mCG117547.1, sequence:GGGTGAAGACAGAAACAAGGTC; Reverse Primer - name:163522_N_SP6_cDNA_mCG117547.1, sequence:CGCCTTTACCTGTCAGCCTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8839 same embryo
 EMAGE:8842 same embryo
 EMAGE:8841 same embryo
 EMAGE:8840 same embryo
 EMAGE:8837 same embryo
 EurExpress:euxassay_012996 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS