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EMAGE:8871

Tmem173 transmembrane protein 173 ( MGI:1919762)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871
"Pseudo-wholemount" of euxassay_010136. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010136_01 euxassay_010136_02 euxassay_010136_03 euxassay_010136_04
EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871
euxassay_010136_05 euxassay_010136_06 euxassay_010136_07 euxassay_010136_08 euxassay_010136_10
EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871
euxassay_010136_11 euxassay_010136_12 euxassay_010136_13 euxassay_010136_14 euxassay_010136_15
EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871
euxassay_010136_16 euxassay_010136_17 euxassay_010136_18 euxassay_010136_19 euxassay_010136_20
EMAGE:8871 EMAGE:8871 EMAGE:8871 EMAGE:8871
euxassay_010136_21 euxassay_010136_22 euxassay_010136_23 euxassay_010136_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8871Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8871_wholemount_strong.wlz
8871_wholemount_moderate.wlz
8871_wholemount_weak.wlz
8871_wholemount_possible.wlz
8871_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8871_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32053
Entity Detected:Tmem173, transmembrane protein 173 ( MGI:1919762)
Sequence:sense strand is shown

>T32053
CATGCTCAGTGGTGCAGGGAGCCGAAGACTGTACATCCTCTTTCCATTGGACTGTGGGGTGCCTGACAAC
CTGAGTGTAGTTGACCCCAACATTCGATTCCGAGATATGCTGCCCCAGCAAAACATCGACCGTGCTGGCA
TCAAGAATCGGGTTTATTCCAACAGCGTCTACGAGATTCTGGAGAACGGACAGCCAGCAGGCGTCTGTAT
CCTGGAGTACGCCACCCCCTTGCAGACCCTGTTTGCCATGTCACAGGATGCCAAAGCTGGCTTCAGTCGG
GAGGATCGGCTTGAGCAGGCTAAACTCTTCTGCCGGACACTTGAGGAAATCCTGGAAGATGTCCCCGAGT
CTCGAAATAACTGCCGCCTCATTGTCTACCAAGAACCCACAGACGGAAACAGTTTCTCACTGTCTCAGGA
GGTGCTCCGGCACATTCGTCAGGAAGAAAAGGAGGAGGTTACCATGAATGCCCCCATGACCTCAGTGGCA
CCTCCTCCCTCCGTACTGTCCCAAGAGCCAAGACTCCTCATCAGTGGTATGGATCAGCCTCTCCCACTCC
GCACTGACCTCATCTGAGGCATGGGACAGCCTTGTCTGGGCTCTAGTGATCCTTTAGCCTCCTGACTGAG
CCTTCCTTCAATGGTTGGGGGCCTCAGAGACTTCACATCTCCAGATGAGTCCCACATTCCTGGGCAAGCC
ATTTATTTCACCTCTCTGAGCCTCAACCAACCCTACTATGAAAGGAGGTCATAATGCGTTCCCTGCCCAG
CCAAAGGATTTTATATATGTAGAAGTTGGTGTCAATGCCTGGTAAACTTGAGAGAAAGGCCAAGTACTTC
CCGTGGATGCTGCAGACATTCCCTGCTCTCTGTTGACCTGTGTGGATGGTACCAGCAGACTTCCAACCCT
CCAGCTTCTGGTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5067343), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 57874. Forward Primer - name:057874_F_IRAV88_f06_2610307O08Rik, sequence:CATGCTCAGTGGTGCAGG; Reverse Primer - name:057874_R_SP6_IRAV88_f06_2610307O08Rik, sequence:TGACCAGAAGCTGGAGGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8867 same embryo
 EMAGE:8869 same embryo
 EMAGE:8870 same embryo
 EMAGE:8872 same embryo
 EMAGE:8868 same embryo
 EurExpress:euxassay_010136 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS