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EMAGE:8875

Arhgef25 Rho guanine nucleotide exchange factor (GEF) 25 ( MGI:1277173)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875
"Pseudo-wholemount" of euxassay_010079. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010079_01 euxassay_010079_02 euxassay_010079_03 euxassay_010079_04
EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875
euxassay_010079_05 euxassay_010079_06 euxassay_010079_07 euxassay_010079_08 euxassay_010079_09
EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875
euxassay_010079_10 euxassay_010079_11 euxassay_010079_12 euxassay_010079_13 euxassay_010079_14
EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875
euxassay_010079_15 euxassay_010079_16 euxassay_010079_17 euxassay_010079_18 euxassay_010079_19
EMAGE:8875 EMAGE:8875 EMAGE:8875 EMAGE:8875
euxassay_010079_20 euxassay_010079_21 euxassay_010079_22 euxassay_010079_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8875Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8875_wholemount_strong.wlz
8875_wholemount_moderate.wlz
8875_wholemount_weak.wlz
8875_wholemount_possible.wlz
8875_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8875_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 08 21 22 23
nasal septum epithelium
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 09 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32126
Entity Detected:Arhgef25, Rho guanine nucleotide exchange factor (GEF) 25 ( MGI:1277173)
Sequence:sense strand is shown

>T32126
TGCTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTGAGGCAGCAGCTGGGACACCGACTGCAGCTCAA
TGACCTCCTCATTAAACCAGTGCAGCGAATCATGAAATACCAGTTGCTGCTGAAGGATTTTCTAAAGTAT
TACAGAAGAGCTGGGAAGGATACTGAAGAGCTGGAGCAAGCTGTGGAGGTCATGTGTTTCGTACCTAAGC
GATGCAATGATATGATGTCCCTGGGGAGACTTCGGGGATTTGAGGGAAAACTGACTGCTCAGGGGAAGCT
CTTGGGCCAGGACACATTCTTGGTTACAGAGCCTGAAGCTGGGGGTCTGCTTTCTTCCCGGGGTCGGGAG
AGGCGTGTCTTCCTGTTCGAGCAAATCATCATCTTCAGTGAGGCCCTGGGAGGAGGAGGCCGAGGTGGCG
CGCAGCCTGGCTACGTGTACAAGAACAGCATCAAGGTGAGCTGCCTAGGGCTGGAGGGGAATCTCCAAGG
CAACCCTTGCCGGTTTGCGCTGACCTCTAGAGGGCCAGAAGGTGGGATCCAGCGCTACGTTCTGCAGGCT
TCAGACCCTGCTGTCAGTCAAGCCTGGATAAAGCAAGTGGCCCAGATCCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6439728), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59264. Forward Primer - name:059264_F_IRAV94_c10_D10Ertd610e, sequence:TGCTGTCAGAGTTTGGGGA; Reverse Primer - name:059264_R_SP6_IRAV94_c10_D10Ertd610e, sequence:CAGGATCTGGGCCACTTG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8878 same embryo
 EMAGE:8873 same embryo
 EMAGE:8877 same embryo
 EMAGE:8874 same embryo
 EMAGE:8876 same embryo
 EurExpress:euxassay_010079 same experiment
 MGI:4824189 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS