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EMAGE:8885

Pde4b phosphodiesterase 4B, cAMP specific ( MGI:99557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885
"Pseudo-wholemount" of euxassay_010161. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010161_01 euxassay_010161_02 euxassay_010161_03 euxassay_010161_04
EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885
euxassay_010161_05 euxassay_010161_06 euxassay_010161_07 euxassay_010161_08 euxassay_010161_09
EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885
euxassay_010161_10 euxassay_010161_11 euxassay_010161_12 euxassay_010161_13 euxassay_010161_14
EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885 EMAGE:8885
euxassay_010161_15 euxassay_010161_16 euxassay_010161_17 euxassay_010161_18 euxassay_010161_19
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euxassay_010161_20 euxassay_010161_21 euxassay_010161_22 euxassay_010161_23 euxassay_010161_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:8885Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
8885_wholemount_strong.wlz
8885_wholemount_moderate.wlz
8885_wholemount_weak.wlz
8885_wholemount_possible.wlz
8885_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:8885_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
weak weak
regionalweak expression: see section 21 22
cochlea
weak weak
regionalweak expression: see section 06 07 08 09 17 18 19
utricle
weak weak
regionalweak expression: see section 04 05 06 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32084
Entity Detected:Pde4b, phosphodiesterase 4B, cAMP specific ( MGI:99557)
Sequence:sense strand is shown

>T32084
TGTCTTGCCAGTCTCCCAGTATGCTCCTCTTGCTCTGAAGTGCTCCAGGATTGAAACCACAGCTTCCCAA
ATTAGCCTGGGAAGAGTGTGCGGACCCAGCAGCCTTTTAACCCGCGTCAGTGCCTTTGCTATGTTCAAGA
CTGCTGTTTTGGATGGTGAATGCTAGCTAGCACTCCATACGAGACATGACAGCAAAAAATTCTCCAAAAG
AATTTACTGCTTCGGAATCTGAGGTTTGCATAAAGACTTTCAAGGAGCAGATGCGCTTGGAACTTGAGCT
TCCAAAGCTACCAGGAAACAGACCTACATCTCCCAAAATTTCTCCACGCAGTTCACCAAGGAATTCACCA
TGCTTTTTCAGAAAGTTGCTGGTGAATAAAAGCATCCGACAGCGGCGTCGCTTCACGGTGGCTCATACAT
GCTTTGATGTGGAAAATGGCCCTTCTCCAGGTCGGAGCCCACTGGACCCTCAAGCCGGCTCTTCGTCGGG
ACTGGTACTTCATGCCGCCTTTCCTGGGCACAGCCAGCGCAGGGAGTCGTTCCTCTACAGATCTGACAGC
GACTATGACTTGTCACCAAAAGCGATGTCCAGGAACTCATCACTTCCCAGTGAGCAACACGGCGATGACC
TGATTGTCACTCCTTTTGCCCAGGTTCTTGCCAGCTTGCGAAGTGTAAGAAACAACTTCACCCTGCTGAC
GAACCTTCATGGAGCGCCGAACAAGAGGTCACCAGCG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4954751), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58082. Forward Primer - name:058082_F_IRAV90_c06_Pde4b, sequence:TGTCTTGCCAGTCTCCCAG; Reverse Primer - name:058082_R_SP6_IRAV90_c06_Pde4b, sequence:CCGCTGGTGACCTCTTGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:8890 same embryo
 EMAGE:8887 same embryo
 EMAGE:8889 same embryo
 EMAGE:8888 same embryo
 EMAGE:8886 same embryo
 EurExpress:euxassay_010161 same experiment
 MGI:4827119 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS