EMAGE mouse gene expression spatial database

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EMAGE:9021

Tspan2 tetraspanin 2 ( MGI:1917997)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012916. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012916_01	euxassay_012916_02	euxassay_012916_03	euxassay_012916_04

euxassay_012916_05	euxassay_012916_06	euxassay_012916_07	euxassay_012916_08	euxassay_012916_09

euxassay_012916_10	euxassay_012916_11	euxassay_012916_12	euxassay_012916_13	euxassay_012916_14

euxassay_012916_15	euxassay_012916_16	euxassay_012916_17	euxassay_012916_18	euxassay_012916_19

euxassay_012916_20	euxassay_012916_21	euxassay_012916_22	euxassay_012916_23	euxassay_012916_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9021_wholemount_strong.wlz

9021_wholemount_moderate.wlz

9021_wholemount_possible.wlz

9021_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9021_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

moderate

regional

moderate expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38673
Entity Detected:	Tspan2, tetraspanin 2 ( MGI:1917997)
Sequence:	sense strand is shown >T38673 TTGTTTTCTCCCTCTTCTCTGCCAACTCTGCTTTGAACTAGTGAAGGCCTCGCCTTCCCACAAGGCCTTT CCTTCACCCTCCTTACTGTTTTTTTCAGATGTGGTGACAGAGGAAAGCAGGCACTGTGCAGCGTGACAGT GGGGGACTCTGGCTGGCACTGTCTGTACCTCTGGAATACCTCATGACTTGAGTTTACATACTTTCCTAAT ATTTTTCCATTTTTCTTATATATCTGTGCAGAGAATTCTTTATAGATTAAGAACATAGTTTTATTCTTTA AAATAAATAAATCGCCCCATAACTCAGATATTATGTTATGAGTACCCTGTCTAAACCAATATAAACACCT TCTTCAATATGTATGCACACAACAAAGGCAAGTTAATGGATCAAAAGTACTTTCGTGCATACAGCTGCAG CACAACTATATTCTTTCTCAGCGAACCCCTGTGGGGATGAATAGATGCTCAGGTTCTTTGGGAGCCTGAG TGATTGATTTTAAGTGGGGAAACCCAGGCACTGCTCGGCCTTTTGGCAGCAAGTGGGAAACCCCACAGCA GGGTCGAAATTAATGTATTTGTCATAATGGAACAGCGTATAAAGGAGTCTTAGGATTATATTGACCACAT AAAAGTGAAGGCATGAGGAATTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 154947. Forward Primer - name:154947_F_cDNA_TC1563420, sequence:TTGTTTTCTCCCTCTTCTCTGC; Reverse Primer - name:154947_N_SP6_cDNA_TC1563420, sequence:GAATTCCTCATGCCTTCACTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9022 same embryo
	EMAGE:9026 same embryo
	EMAGE:9024 same embryo
	EMAGE:9025 same embryo
	EMAGE:9023 same embryo
	EurExpress:euxassay_012916 same experiment
	MGI:4828964 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source