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EMAGE:9129

Pde6d phosphodiesterase 6D, cGMP-specific, rod, delta ( MGI:1270843)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129
"Pseudo-wholemount" of euxassay_018591. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018591_01 euxassay_018591_02 euxassay_018591_03 euxassay_018591_04
EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129
euxassay_018591_05 euxassay_018591_06 euxassay_018591_07 euxassay_018591_08 euxassay_018591_09
EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129
euxassay_018591_10 euxassay_018591_11 euxassay_018591_12 euxassay_018591_13 euxassay_018591_14
EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129 EMAGE:9129
euxassay_018591_15 euxassay_018591_16 euxassay_018591_17 euxassay_018591_18 euxassay_018591_19
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euxassay_018591_20 euxassay_018591_21 euxassay_018591_22 euxassay_018591_23 euxassay_018591_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9129Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9129_wholemount_strong.wlz
9129_wholemount_moderate.wlz
9129_wholemount_weak.wlz
9129_wholemount_possible.wlz
9129_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9129_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50822
Entity Detected:Pde6d, phosphodiesterase 6D, cGMP-specific, rod, delta ( MGI:1270843)
Sequence:sense strand is shown

>T50822
TCCTGGGTACCGTCTGCGAGGCCCCGCGGAGCCAGCGTGGGAGGGCCGCGGGCGGCAGGCGCCGCATCAT
GTCAGCCAAGGACGAGCGGGCCAGGGATATCCTGAGAGGCTTCAAACTAAATTGGATGAACCTTCGGGAT
GCCGAAACAGGGAAGATACTCTGGCAAGGAACAGAAGACCTGTCTGTCCCTGGTGTGGAACATGAAGCCC
GTGTGCCCAAGAAAATCCTCAAGTGCAAGGCAGTGTCTCGAGAATTGAACTTTTCTTCAGCAGAACAAAT
GGAAAAATTCCGCCTGGAACAAAAAGTTTACTTCAAAGGACAATGCCTAGAAGAGTGGTTCTTCGAGTTT
GGCTTTGTGATCCCTAACTCCACAAACACCTGGCAGTCCTTGATAGAAGCAGCGCCTGAGTCCCAGATGA
TGCCCGCCAGCGTCCTAACGGGCAATGTCATCATAGAGACAAAGTTCTTTGACGATGATCTTCTTGTCAG
CACATCCAAAGTGAGGCTTTTCTATGTTTGAGAGAATTTGTGTGCACTTCTAAGAATTTGGCCGGGGGCA
GGAGGAAGCTGAACTTGTTCTCTGCACACCTGACTGGGATACTCCAGCTGACTCACTCCTTCAACACAGA
ACCCACCTGTGCACCAGGAAACCAGCTCTGAATAGACGGCCAGTGGCTTTTCTCCCAAAACCTCCATCTT
CACTGACAAGACTAAACTCCCAACCCCAGCCAAAGGCAGGGATAAGCCTCGACTCCTTCCTGGCGCAAAC
ATGGGAACAAACCCTCCCAGGAGCCACGCTGCGCCTGTCACCTGCCTGCCTCCTCCTTGGGCCCCACAGG
CTTTCTTTAGCCT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCCTGGGTACCGTCTGCGAG; Reverse Primer - name:unspecified, sequence:AGGCTAAAGAAAGCCTGTGG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9130 same embryo
 EurExpress:euxassay_018591 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS