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EMAGE:9210

Dlx5 distal-less homeobox 5 ( MGI:101926)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210
"Pseudo-wholemount" of euxassay_017956. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017956_01 euxassay_017956_02 euxassay_017956_03 euxassay_017956_04
EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210
euxassay_017956_05 euxassay_017956_06 euxassay_017956_07 euxassay_017956_08 euxassay_017956_09
EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210
euxassay_017956_10 euxassay_017956_11 euxassay_017956_12 euxassay_017956_13 euxassay_017956_14
EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210
euxassay_017956_15 euxassay_017956_16 euxassay_017956_17 euxassay_017956_18 euxassay_017956_19
EMAGE:9210 EMAGE:9210 EMAGE:9210 EMAGE:9210
euxassay_017956_20 euxassay_017956_21 euxassay_017956_22 euxassay_017956_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9210Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9210_wholemount_strong.wlz
9210_wholemount_moderate.wlz
9210_wholemount_weak.wlz
9210_wholemount_possible.wlz
9210_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9210_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50313
Entity Detected:Dlx5, distal-less homeobox 5 ( MGI:101926)
Sequence:sense strand is shown

>T50313
CAGGTGAAAATCTGGTTTCAGAACAAAAGATCCAAGATCAAGAAGATCATGAAAAACGGGGAGATGCCCC
CGGAGCACAGTCCCAGCTCCAGCGACCCTATGGCGTGTAACTCGCCACAGTCACCAGCGGTGTGGGAGCC
CCAGGGCTCATCCCGCTCTCTCAGCCACCACCCTCATGCCCACCCTCCGACCTCCAACCAGTCCCCCGCG
TCCAGCTACCTGGAGAACTCGGCTTCCTGGTACCCAAGCGCAGCCAGCTCAATCAATTCCCACCTGCCAC
CGCCGGGCTCCTTGCAGCACCCGCTGGCACTGGCCTCCGGGACGCTTTATTAGATGGGCTACTTTCTCTT
GCTCAGTTTTGGGACTACAGTGTTGTGCTGGTTTAGAAATCAGAAAGAAAGGAATTCATACGGGGATGTT
TGAACAATGGAAACAAAAAAGATTCGTGTGTAAACTTTTTTTTTGCATGTAAGTTATTGCATTTCAGAGA
GCCCCGCCCCCTTTTTTACGAAAAGAACCCCTTTTTTTTTTTTTTTTTTGCGCAACTGTGGACACTATCA
ATGGTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGGTGAAAATCTGGTTTCAGAAC; Reverse Primer - name:unspecified, sequence:CACCATTGATAGTGTCCACAGTTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9211 same embryo
 EMAGE:9208 same embryo
 EMAGE:9209 same embryo
 EurExpress:euxassay_017956 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS