Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9314

Pde4dip phosphodiesterase 4D interacting protein (myomegalin) ( MGI:1891434)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314
"Pseudo-wholemount" of euxassay_017954. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017954_01 euxassay_017954_02 euxassay_017954_03 euxassay_017954_04
EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314
euxassay_017954_05 euxassay_017954_06 euxassay_017954_07 euxassay_017954_08 euxassay_017954_09
EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314
euxassay_017954_10 euxassay_017954_11 euxassay_017954_12 euxassay_017954_13 euxassay_017954_14
EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314
euxassay_017954_15 euxassay_017954_16 euxassay_017954_17 euxassay_017954_18 euxassay_017954_19
EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314 EMAGE:9314
euxassay_017954_20 euxassay_017954_21 euxassay_017954_22 euxassay_017954_23 euxassay_017954_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9314Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9314_wholemount_strong.wlz
9314_wholemount_moderate.wlz
9314_wholemount_weak.wlz
9314_wholemount_possible.wlz
9314_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9314_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50972
Entity Detected:Pde4dip, phosphodiesterase 4D interacting protein (myomegalin) ( MGI:1891434)
Sequence:sense strand is shown

>T50972
CAGGGCTGGAGAAAGAACTGAGCAACGCCAAGGAGGAGCTCGAGCTCATGGCCAAAAAAGAAAGAGAAAG
CCAGATGGAACTGTCTGCCCTGCAGTCCATGATGGCCATGCAAGAGGAAGAGCTGCAGGTGCAGGCTGCT
GACTTGGAGTCCCTGACCAGGAATGTGCAGATAAAAGAAGATCTCATAAAGGACCTGCAGATGCAACTGG
TCGACCCTGAAGATATACCAGCCATGGAGCGTCTTACCCAAGAGGTCTTACTTCTTCGGGAAAAAGTTGC
TTCCGTGGAACCCCAGGGTCAGGAAGTATCAGGGAACAAGAGACAGCAGTTGCTGCTGATGTTAGAAGGA
CTAGTGGATGAACGGAGTCGGCTCAACGAGGCCCTGCAAGCTGAGAGGCAACTCTACAGCAGCCTGGTCA
AGTTCCATGCCCAGCCAGAGAACTCTGAGAGAGACGGAACTCTGCAGGTGGAACTGGAAGGGGCCCAGGT
GTTACGCACTCGACTAGAAGAAGTTCTTGGAAGAAGCCTGGAGCGTTTAAGCAGGCTGGAGAGCCTGGCC
GCCATTGGAGGTGGGGAACTGGAAAGTGTGCAAGCCCGTCACAAGCATGCCTTCTGAGCACTGGCGGGTC
ACACTGCGACCCAGGATGGAAACCCTGTTTATACTAACCAGAAAGCTGTGTCGGACTTGACAACCAAGTG
TGGCTTACTAATGGTAACGGTAGGACTGCTGTCAGTAGCAGCAAAGGGAAGCAGCTGGAGTCTTCCTTAC
TCCCTGCACGGACCTAGTTCTTAGCTAACTGAGGTCTTGAAGCATACTGCACATCTTAAATCATTCCATT
TTAATTTCCCATTCATACTGCTCTTCTCCCTGGCCTGTCCTCATTCCTTTACCCCTTCCATGGGCCAATA
AAGTTTACTCCCCCATCTTACCACCCCCGCCACAAGTCCCACGTCATGT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGGGCTGGAGAAAGAACTG; Reverse Primer - name:unspecified, sequence:ACATGACGTGGGACTTGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9311 same embryo
 EMAGE:9310 same embryo
 EMAGE:9312 same embryo
 EMAGE:9313 same embryo
 EurExpress:euxassay_017954 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS