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EMAGE:9329

Slc36a4 solute carrier family 36 (proton/amino acid symporter), member 4 ( MGI:2442595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329
"Pseudo-wholemount" of euxassay_018029. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018029_01 euxassay_018029_02 euxassay_018029_03 euxassay_018029_04
EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329
euxassay_018029_05 euxassay_018029_06 euxassay_018029_07 euxassay_018029_08 euxassay_018029_09
EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329
euxassay_018029_10 euxassay_018029_11 euxassay_018029_12 euxassay_018029_13 euxassay_018029_14
EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329 EMAGE:9329
euxassay_018029_15 euxassay_018029_16 euxassay_018029_17 euxassay_018029_18 euxassay_018029_19
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euxassay_018029_20 euxassay_018029_21 euxassay_018029_22 euxassay_018029_23 euxassay_018029_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9329Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9329_wholemount_strong.wlz
9329_wholemount_moderate.wlz
9329_wholemount_weak.wlz
9329_wholemount_possible.wlz
9329_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9329_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51016
Entity Detected:Slc36a4, solute carrier family 36 (proton/amino acid symporter), member 4 ( MGI:2442595)
Sequence:sense strand is shown

>T51016
TACATCAGCCTAGCCACTTTAGGCTACATGTGTTTCCGTGACGAGATCAAAGGCAGCATTACACTGAATC
TTCCCCAGGACATGTGGTTGTATCAGTCAGTGAAAATTCTGTATTCCTTTGGCATTTTTGTGACCTATTC
AATTCAGTTCTATGTCCCAGCAGAGATCATTATCCCTGGAGTCACTGCTAGACTTCATGCCAAATGGAAG
CGCATTTGTGAATTTGGGATACGGTCCCTCTTGGTTAGTATCACCTGTGCTGGGGCGATTCTCATTCCTC
GCCTAGACATTGTGATCTCCTTCGTGGGGGGCTGTGAGCAGCAGTACACTAGCCCTGATCCTGCCCCCAC
TTGTGGAAATCCTTACATTTTCTAAGGACCACTACAACATATGGATGATCCTGAAAAACATTTCCATAGC
GTTCACTGGAGTCGTGGGCTTCTTGTTGGGCACCTATGTCACTGTTGAAGAAATTATTTATCCGACTACG
GCAGTTGTTGCTGGCACCTCCCAGAGTCCCTTTCTGAATGTGAACTCTACATGCATAACAAGTGGTTTGT
AATCATGGGAGAAGAGCGGTGAACCCTCTCCTGTCACCCCAATTCTAACAGCAGTCAAGAACGACTACAG
TGCTTCCCCATACACTGAACAGTGCCAAACTCCTAGTTTTTGTTGGCAGAGTATTGCAATTTGACTGTGA
ACTGGACATCTTTTGCAGCTATGATAAACTATGGAAGATTATTGCTTTTATCTAATGACAATGAAAAATT
GAAAAAATTTAAAATTCAAAATGTAAACAATAAGCACAGCCCATTATTGTGTTTCTTTAAGAAACACTTT
GAGTAAGAGTTGTGTTTCTTACCACTATTGAGAAGATAAAGGATTTCAACATGGTATAAATTAAATAGGC
ACATGGTTTAAAAACCTCTTTGAAGCACCCCACAATAGCAAAGTACACCTCCTGTGTATGTGCACA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TACATCAGCCTAGCCACTTTAGG; Reverse Primer - name:unspecified, sequence:TGTGCACATACACAGGAGGTGTAC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9331 same embryo
 EMAGE:9328 same embryo
 EMAGE:9332 same embryo
 EMAGE:9330 same embryo
 EurExpress:euxassay_018029 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS