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EMAGE:9397

Prkca protein kinase C, alpha ( MGI:97595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397
"Pseudo-wholemount" of euxassay_018262. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018262_01 euxassay_018262_02 euxassay_018262_03 euxassay_018262_04
EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397
euxassay_018262_05 euxassay_018262_06 euxassay_018262_07 euxassay_018262_08 euxassay_018262_09
EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397
euxassay_018262_10 euxassay_018262_11 euxassay_018262_12 euxassay_018262_13 euxassay_018262_14
EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397 EMAGE:9397
euxassay_018262_15 euxassay_018262_16 euxassay_018262_17 euxassay_018262_18 euxassay_018262_19
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euxassay_018262_20 euxassay_018262_21 euxassay_018262_22 euxassay_018262_23 euxassay_018262_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9397Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9397_wholemount_strong.wlz
9397_wholemount_moderate.wlz
9397_wholemount_weak.wlz
9397_wholemount_possible.wlz
9397_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9397_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50882
Entity Detected:Prkca, protein kinase C, alpha ( MGI:97595)
Sequence:sense strand is shown

>T50882
CCATCAGTGGGAAGTGATCCTTAACCATAAAAATTTAAGGCTATGGCTTTGTATTTTGTTCCACACAGAG
GCCTGGAAATTTTAGGGACATTAGTAATACATTATCAACTTTTTTCCTCACCCCCCAACCAAAAGCATTA
TCTTAGTGGATGATGACATAAAGTACAGAGTATAGTTTAATTATGTAGAAGTCATGTCCGGCTACAAGTT
GATTCTTCCTAAAAATCAAAGAGACTTGGACCCTATTTTTTGGTACGATTTGATATATTCTCCATACTTT
CCATTTCTTGGATTTTCAGTATCAGTATCAACCAGAGATAGTAAAGTGACCCACCCTAACTCAAGGAACA
CTTCCACCCAAGATGTCTTTGGAACAAAGACAGGAAGTCCAAAGAGAAAGCAAAGAGAGGTTGGGGGCTC
ATATATTCAAGACCACATAGCTTCTATTCTTCGTTTCAATGGAAACATCCCTAGACTCAGAGAGGGCAGG
ATGAACCTGATCACTGTTCCCAGTCATCATAGCACAACCATAGTGCACAGTTTAAGAATGAAAGAAAACT
TCAGACAAATATTCACTGAATCTACCATCTGTAATCCCCTGCTTAGTTGATAACAATCTCTATAACTCAT
TCTTTTTAAGAGGCCAAATCATCTAAGGGCTTTGCTAAACAAGCATATGAAATCATTTCAGATCAAGGAT
AAACCATGTGTACGTTCATTCTAATCTCTGGGAGATGACTCTTCAATCCAGGGTGCCATCAGTAATCATG
CCACTGTTCACGA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCATCAGTGGGAAGTGATCC; Reverse Primer - name:unspecified, sequence:TCGTGAACAGTGGCATGATTA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9398 same embryo
 EMAGE:9400 same embryo
 EMAGE:9396 same embryo
 EMAGE:9399 same embryo
 EurExpress:euxassay_018262 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS