Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9487

Ctnna1 catenin (cadherin associated protein), alpha 1 ( MGI:88274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487
"Pseudo-wholemount" of euxassay_018188. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018188_01 euxassay_018188_02 euxassay_018188_03 euxassay_018188_04
EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487
euxassay_018188_05 euxassay_018188_06 euxassay_018188_07 euxassay_018188_08 euxassay_018188_09
EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487
euxassay_018188_10 euxassay_018188_11 euxassay_018188_12 euxassay_018188_13 euxassay_018188_14
EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487
euxassay_018188_15 euxassay_018188_16 euxassay_018188_17 euxassay_018188_18 euxassay_018188_19
EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487 EMAGE:9487
euxassay_018188_20 euxassay_018188_21 euxassay_018188_22 euxassay_018188_23 euxassay_018188_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9487Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9487_wholemount_strong.wlz
9487_wholemount_moderate.wlz
9487_wholemount_weak.wlz
9487_wholemount_possible.wlz
9487_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9487_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50212
Entity Detected:Ctnna1, catenin (cadherin associated protein), alpha 1 ( MGI:88274)
Sequence:sense strand is shown

>T50212
CGCAGACCAAGATTAAACGAGCTTCTCAGAAGAAACACGTGAACCCAGTGCAGGCCCTGAGCGAGTTCAA
AGCCATGGACAGCATCTGAGCCTGCACAGCCCATGGGGTTCTGAAAAAAACCAGTCACATGCTTCACTCA
AATGTATTTGCTAAGTAGAGAACACTGTCTTATTCCACAGGGAGGTAGCAGATTTCAGACTCAACCAGGT
GGTGAATTTTGCAAGGACATGTTTGTGTTAAAGCTGGTTAGAAAAAAAAAGCTTTTAGAATTCAAAAATA
TATTTCTAGCTATATTTTTATAAGTTTAATGAAAATAACAGTTCTGTAACCAAAGAGTACCACATTAACT
TCTTGGTCACATTTCTGAGTAGGCTGAAGTGCCTGCTTTCTAGGTAGGGGTGAGCGCCTGAGGGGAGAGG
TTGCTTTTTAGCCCTACTGTTGGGCAGATGGAATTAAATGACCAAGGGTGGCCCAGTGTAAATCACAGGG
CACCTTAGACGGTCTGGAGAAGGAAGGTACCAGAAACCTCTTCTGAGGAATAAACTATAGGAGACAATAA
AATATAACACAGTACCCAGGAAGTTGATTGCTTTCTTTATGAAGCAGAAGGGGAATTATGACGCATGGCT
ACAGTTACTAATGGACTCTGCTGCAGAACATTAATGGAGTTGCTTTATTCGGGCTCGTGTGTTGTGATGC
TGTAATCAGAACATGCTTCCCTGTTATTCCAGCTTGAAATGCAAATTCACCATCGGGCTCATTTCTAATC
GTCGCAGTGTTTCCTGCTTTGGGCCAGCAAAGAAGCCTGATGACTGCATAGGGTTTGCTTAGGCAATACT
TAGACGCACCGTGTTTGTG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CGCAGACCAAGATTAAACGAG; Reverse Primer - name:unspecified, sequence:CACAAACACGGTGCGTCTAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9490 same embryo
 EMAGE:9488 same embryo
 EMAGE:9489 same embryo
 EMAGE:9491 same embryo
 EurExpress:euxassay_018188 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS