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EMAGE:9497

Akap8 A kinase (PRKA) anchor protein 8 ( MGI:1928488)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497
"Pseudo-wholemount" of euxassay_018081. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018081_01 euxassay_018081_02 euxassay_018081_03 euxassay_018081_04
EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497
euxassay_018081_05 euxassay_018081_06 euxassay_018081_07 euxassay_018081_08 euxassay_018081_09
EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497
euxassay_018081_10 euxassay_018081_11 euxassay_018081_12 euxassay_018081_13 euxassay_018081_14
EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497 EMAGE:9497
euxassay_018081_15 euxassay_018081_16 euxassay_018081_17 euxassay_018081_18 euxassay_018081_19
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euxassay_018081_20 euxassay_018081_21 euxassay_018081_22 euxassay_018081_23 euxassay_018081_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9497Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9497_wholemount_strong.wlz
9497_wholemount_moderate.wlz
9497_wholemount_weak.wlz
9497_wholemount_possible.wlz
9497_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9497_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50087
Entity Detected:Akap8, A kinase (PRKA) anchor protein 8 ( MGI:1928488)
Sequence:sense strand is shown

>T50087
TTGCCAATGAAACTGCTGACCTTGAGACAGAAGGAGATGAAAATGTAGGAGAGGAGAAGGAGGAGACACC
AGAGGAGGTAGCTGCCGAAGTCTTAGCAGAGGTGATCACAGCAGCAGTGAAGACTGTAGAGGGGGAAGGA
GGACCTGCTGCAGCGCATAGTGACGTCCTAACTGAAGTGGAAGGGCCTGTGGACACAGCAGAGGCCAGCA
GTGACCCCCACACTGAAAAGCTACTAGAAGAGCAGACCTGTGAAGCAGCATCTGAAACCAGGAGCATCGA
AGACAAGACCAGAGGTGAGGCTGCTGAGGCCAGAAACGAAGCAGCTATGCCAACAGCAGACGCAGGAAGC
ACGTTACCTGTCATAGCAATCCCGGGAATCATGGAAGATGAGCTGGAACAAACTGGTGCAGAGGCCAAAG
ATATTCCCACCGAATGATTTTTTTCTTCCCTGTTTCAAGGGATATGTTATATATCATGTGTTCTTTTTAT
TTTATAAGATGTACTAGGGTGTGTATTATTTGGTGGAAAGACTGGGCCATTTCCTTCCCAATGTACCTCA
AGGATTGATGCTATACAGTAGATGGCTTCCTACCTCTGTTAGAATACAAAAAGAGGTAAACCATTTTCCC
AAGTGGCCTTTGATGGCTATCTGTGCACTGCAGTTAGAATAATAAAAGTAGATCTTCCTGGTAATTGTTA
AGTCCTAAAACGGACAGAATGTGAGGATTTTTTTGTGTGTGTGTTATGTTTTTGTTTGGTTGGTCTTTGT
TTCGTTTTATTTGCTTTTTCTCCTGGAAAGCAATTTGTTACAAATATAACTTGTTTGAGGGGGAAAAAAT
TAATTATTTAGGGGAATTCCCTTATTCACATATGAATTTATTCATAAATTATGCATGGTTGATGTGGGAC
ACACACGATTCAACCATCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TTGCCAATGAAACTGCTGAC; Reverse Primer - name:unspecified, sequence:GATGGTTGAATCGTGTGTG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9495 same embryo
 EMAGE:9496 same embryo
 EMAGE:9494 same embryo
 EMAGE:9493 same embryo
 EMAGE:9492 same embryo
 EurExpress:euxassay_018081 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS