EMAGE mouse gene expression spatial database

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EMAGE:9507

Idh3a isocitrate dehydrogenase 3 (NAD+) alpha ( MGI:1915084)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_018330. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_018330_01	euxassay_018330_02	euxassay_018330_03	euxassay_018330_04

euxassay_018330_05	euxassay_018330_06	euxassay_018330_07	euxassay_018330_08	euxassay_018330_09

euxassay_018330_10	euxassay_018330_11	euxassay_018330_12	euxassay_018330_13	euxassay_018330_14

euxassay_018330_15	euxassay_018330_16	euxassay_018330_17	euxassay_018330_18	euxassay_018330_19

euxassay_018330_20	euxassay_018330_21	euxassay_018330_22	euxassay_018330_23	euxassay_018330_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9507_wholemount_strong.wlz

9507_wholemount_moderate.wlz

9507_wholemount_weak.wlz

9507_wholemount_possible.wlz

9507_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9507_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T50546
Entity Detected:	Idh3a, isocitrate dehydrogenase 3 (NAD+) alpha ( MGI:1915084)
Sequence:	sense strand is shown >T50546 GGGTGTTGCCGCTGTCGCCGCGGTGAGGGAAGTGGACGCGATGGCCGGGTCCGCGTGGGTGTCCAAGGTC TCTCGGCTGCTGGGTGCATTCCACAACACAAAACAGGTGACAAGAGGTTTTGCTGGTGGTGTTCAGACAG TAACTTTAATTCCTGGAGATGGAATTGGCCCAGAAATTTCAGCCTCAGTCATGAAGATTTTTGATGCTGG CCAAAGCACCTATTCAGTGGGAGGAGCGCAATGTCACAGCAATTCAAGGACCAGGAGGAAAGTGGGATGA TCCCTCCAGAAGCCAAGGAGTCCATGGATAAGAACAAGATGGGCTTGAAAGGCCCACTAAAGACCCCAAT AGCCGCTGGCCATCCATCTATGAATCTGTTGCTTCGTAAGACATTTGACCTTTATGCCAATGTCCGGCCA TGTGTCTCAATTGAAGGTTATAAAACCCCTTACACGGATGTAAATATCGTCACCATCCGAGAGAACACGG AAGGAGAATACAGTGGAATTGAGCATGTGATCGTTGATGGGGTTGTGCAGAGCATCAAGCTCATCACCGA AGAAGCAAGCAAGCGCATTGCAGAGTTTGCCTTCGAGTACGCTCGGAACAACCACCGGAGCAACGTCACA GCTGTGCACAAAGCTAACATCATGAGGATGTCAGATGGGCTCTTTCTGCAAAAATGCAGGGAAGTTGCGG AGAACTGTAAAGACATTAAATTTAACGAGATGTACCTTGATACTGTATGTTTAAATATGGTACAAGACCC ATCCCAGTTTGATGTTCTTGTCATGCCAAATTTATACGGAGACATCCTTAGTGATCTGTGTGCAGGACTG ATTGGAGGTCTTGGGGTGACTCCAAGTGGCAATATTGGAGCCAACGGTGTTGCCATCTTTGAATCGGTTC ATGGAACAGCCCCGGACATTGCAGGCAAGGACATGGCCAACCCCACGGCCCTCCTGCTTAGTGCTGTGAT GATGCTTCGCCACATGGGACTTTTTGACCATGCAGCAAAAATCGAGGCTGCATGTTTTGCTACAATTAAG GATGGAAAGAGCTTAACAAAAGATCTGGGAGGCAACGCGAAGTGCTCTGACTTCACAGAAGAAATCTGTC GTAGAGTCAAAGACTTAGATTAGCACTCCTGCTGGTGGATTTGCTGCAGTCAGTCAATCACTCCAAAAGG ATACCCTGTAATCCTCCTTGAGGGCGCCCACCATTGGTTTGCTTGCTTCTTGACAGAGTACGTTTTTTGA ATCTGGCCTTTTCTTAACAAAACCCTTGCAATGGATGCACATGATGGCCCCAGGCCTTCATTCAAAGGGT TTTCCCAAGTGCTGGTTGTATTTATTGTCCGTCTGGTAAACCTTATTTTGTAAACTGTAAGTGAACTGTA TCATTTATCATTGTTAACCCATTTTACACTTCAGGCAAAATCATTTTCCTCAACTGTAAATATTCTGATA CAGAATTAATAAGAGAAGATATTTAACTTTTTAACAAAAGCCCTGGATTTTTGGTTTATGAAAAACAAAC TGGGAATAAAACAGGGTTTCAACAATCGCACAAGATAACATTATTCTAATACTAATGGGTACAAAAGAAA TTTACTGGGAAAGTTCACAGCAAAAAACTGGTATATTTCTTAAAAATATGGAAATAAAGTATTTGTCCTA TACATGAATTACTATTAATAAAAATGTAAGCTCCAAGAAATCCATAATGAATGATGTAATTTTGTTACTA CATCGGTAATCCTTGTCAAGGCCCCGGATGCTCTCTGTGTATTTGATTCTTTGGTTACCTTGAGATTCAC TATTTGGGGGGAAGAGCTTTCAGATAAGGGAGATCACTCCTCACTAGACAGATCGTCAGCATTGCGAGCT GTCAGCCATGAGAGCCAGCCACTGCAGATCCCCTCCCACGTGGCCACACTCCAGCCAGTGCTGCAGGTGA CCCTGGAAAGGCCTGGCTGCCCCTTGACTTTCCCTAAAGC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:unspecified; Reverse Primer - name:unspecified, sequence:unspecified. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9509 same embryo
	EMAGE:9506 same embryo
	EMAGE:9508 same embryo
	EurExpress:euxassay_018330 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source