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EMAGE:9697

Sprr1a small proline-rich protein 1A ( MGI:106660)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697
"Pseudo-wholemount" of euxassay_018436. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018436_01 euxassay_018436_02 euxassay_018436_03 euxassay_018436_04
EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697
euxassay_018436_05 euxassay_018436_06 euxassay_018436_07 euxassay_018436_08 euxassay_018436_09
EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697
euxassay_018436_10 euxassay_018436_11 euxassay_018436_12 euxassay_018436_13 euxassay_018436_14
EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697
euxassay_018436_15 euxassay_018436_16 euxassay_018436_17 euxassay_018436_18 euxassay_018436_19
EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697 EMAGE:9697
euxassay_018436_20 euxassay_018436_21 euxassay_018436_22 euxassay_018436_23 euxassay_018436_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9697Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9697_wholemount_strong.wlz
9697_wholemount_moderate.wlz
9697_wholemount_weak.wlz
9697_wholemount_possible.wlz
9697_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9697_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T51023
Entity Detected:Sprr1a, small proline-rich protein 1A ( MGI:106660)
Sequence:sense strand is shown

>T51023
CAGAGAACCTGCTCTTCTCTGAGTATTAGGACCCAAGTGCTATCTAACCATGAGTTCCCACCAGCAGAAG
CAGCCCTGCACTGTACCTCCTCAGCTGCACCAGCAGCAGGTGAAGCAGCCTTGCCAGCCACCACCCCAGG
AACCTTGTGCCCCCAAAACCAAGGATCCCTGCCACCCTGTTCCTGAGCCCTGCAACCCCAAGGGGCCAGA
GCCCTGCCACCCCAAGGCACCCGAGCCCTGCCACCCCAAGGCACCTGAGCCCTGCAACCCCAAGGTGCCA
GAGCCCTGCCAGCCTAAGGTGCCAGAGCCCTGCCAGCCTAAGGTGCCAGAGCCCTGCAACCCCAAGGTGC
CAGAGCCCTGCCAACCTAAGGCACCAGAGCCTTGCCACCCCAAGGCGCCTGAGCCCTGCCACCCTGTTGT
TCCCGAGCCCTGCCCCTCAACTGTCACTCCATCACCATACCAGCAGAAGACAAAGCAGAAGTAATATTGT
CCAGAGCCATGCCTGAAGACCTGATCACCAGATGCTGAGGCTGCTGTCTATCCTGCTTATGAGTCCCATT
GCCTTGTGCTACCAATGCTGTGACCTTCAGTCTTAATCCCTCTCTCCTTGCACCACCTAAAAAGTTGACT
CTCATCCTCATCTTCAAGGGCTCCTGAGCCTCTTAACATTGCCCAAAGTCATATTGAATGGCTACACTTT
TCATGGCTCAGGATTCATCTGAAGGGGGTGAGGAGTGAGACAAGTGTATGGTCAATATTTTCCCCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CAGAGAACCTGCTCTTCTCTGAGT; Reverse Primer - name:unspecified, sequence:GGGGGAAAATATTGACCATACA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9693 same embryo
 EMAGE:9695 same embryo
 EMAGE:9696 same embryo
 EMAGE:9694 same embryo
 EMAGE:9692 same embryo
 EurExpress:euxassay_018436 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS