Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:9784

Adh4 alcohol dehydrogenase 4 (class II), pi polypeptide ( MGI:1349472)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9784
euxassay_018700_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9784Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9784_wholemount_moderate.wlz
9784_wholemount_weak.wlz
9784_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9784_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50089
Entity Detected:Adh4, alcohol dehydrogenase 4 (class II), pi polypeptide ( MGI:1349472)
Sequence:sense strand is shown

>T50089
TCATGGAAGACAGAACCAGCAGGTTTACCTGCAAAGGAAGATCAATTTACCATTTCATGGGCGTCAGTTC
ATTCTCTCAATACACTGTGGTTTCAGAGGCCAATCTTGCCAGAGTCGATGATGAGGCAAACTTGGAGAGA
GTGTGTCTGATTGGATGTGGGTTCTCATCAGGCTACGGGGCTGCCATCAATACTGCCAAGGTCACCCCCG
GTTCCACTTGTGCTGTCTTTGGCCTGGGGTGTGTAGGTCTTTCTGCCATAATTGGGTGTAAAATAGCAGG
TGCTTCCAGAATCATAGCTATTGACATCAACGGTGAGAAGTTCCCAAAAGCCAAGGCCCTGGGAGCCACT
GACTGCCTGAATCCCAGGGAGCTAGACAAACCTGTCCAGGATGTCATCACTGAGCTGACAGCTGGAGGTG
TGGATTACTCCCTTGACTGTGCAGGAACAGCTCAGACCTTGAAAGCAGCTGTGGACTGCACTGTATTAGG
CTGGGGGTCGTGCACTGTGGTCGGAGCAAAGGTTGACGAAATGACTATACCCACCGTGGACGTTATATTG
GGCCGTTCTATAAATGGAACATTCTTTGGTGGTTGGAAAAGTGTAGACTCTGTCCCAAACCTGGTTTCTG
ACTACAAGAATAAGAAATTCGATCTGGACTTACTGGTGACCCATGCCCTACCTTTCGAGAGCATAAATGA
TGCCATTGACCTGATGAAGGAAGGAAAAAGCATCCGAACAATCCTGACCTTCTGATCCATTGACCTGATG
AAAAGGAAGGAAAATCATCCGAACAATCCTGACCTTCTGAAATGCCAGGAGCAACTTGGAATGTTATCTG
ATTGGATCCGAGCCTGT
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCATGGAAGACAGAACCAGC; Reverse Primer - name:unspecified, sequence:ACAGGCTCGGATCCAATCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9783 same embryo
 EMAGE:9781 same embryo
 EMAGE:9780 same embryo
 EMAGE:9782 same embryo
 EurExpress:euxassay_018700 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS