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EMAGE:9850

Mtor mechanistic target of rapamycin (serine/threonine kinase) ( MGI:1928394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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"Pseudo-wholemount" of euxassay_018764. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_018764_01 euxassay_018764_02 euxassay_018764_03 euxassay_018764_04
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euxassay_018764_20 euxassay_018764_21 euxassay_018764_22 euxassay_018764_23 euxassay_018764_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9850Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9850_wholemount_strong.wlz
9850_wholemount_moderate.wlz
9850_wholemount_weak.wlz
9850_wholemount_possible.wlz
9850_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9850_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50398
Entity Detected:Mtor, mechanistic target of rapamycin (serine/threonine kinase) ( MGI:1928394)
Sequence:sense strand is shown

>T50398
CCACACTGTGATGGAAGTGCTCCGAGAGCACAAGGACAGTGTCATGGCTGTGCTGGAAGCCTTTGTCTAT
GACCCGCTGCTCAACTGGAGGCTGATGGACACGAATACCAAAGGCAATAAGCGGTCCCGGACAAGGACAG
ACTCCTACTCTGCCGGCCAGTCAGTAGAAATTTTGGACGGTGTAGAACTTGGAGAACCAGCCCATAAGAA
AGCAGGGACCACTGTGCCAGAATCCATCCATTCATTCATTGGAGACGGTTTGGTGAAACCAGAAGCCTTA
AACAAGAAAGCTATTCAGATTATTAACAGGGTTCGAGATAAGCTCACTGGTCGGGATTTCTCTCATGATG
ACACTTTGGATGTTCCAACCCAAGTGGAGCTGCTTATCAAGCAGGCAACATCTCACGAGAACCTCTGCCA
GTGCTACATTGGCTGGTGTCCCTTCTGGTAACTGAGGCCTGGAAAACCACGTCGCCTCCTCCCAGGCTTT
AGTACCTTGTCTGTGCTTCCAGTGGACTAAAACCATGGACAGACAGTTGGACTTGTTAAATATTTTGAAA
TGTATATGAAAAGAACTACTGTATATTCAAAGTTGGCTTCCGCCAACCTCCTAGCTGCTGTTGAAAAGAC
ACTGTCAGAAACACAAGGCTTGATTCAGTTCCCAGGACAGTGAAACACAGTAATCCTTCAGAAGCCAAGC
CTTGGATTTTGGGAGAACAGAAGATGGGTAACTGAGAAATACGGGTTTTGACTTAACTTACAAGAAAACT
CATCATAAACATTGCTGACAGAATAATCCAGTTGGTCCTCTCAACCAGGGGCTCCAACGGCAAGGACACA
GAGGTCGGCACTCCACCATCCTGTTACCTCACCCGTCCCTGGATGCAGTGGCGACATTTGCAGGATGGGC
CAACATGGCTAAGAGAGTCTGTCTTCCGCCTGACCCCACGATGCTGAAACTCACAAGACCTGCCC
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CCACACTGTGATGGAAGTGC; Reverse Primer - name:unspecified, sequence:GGGCAGGTCTTGTGAGTTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9849 same embryo
 EMAGE:9853 same embryo
 EMAGE:9851 same embryo
 EMAGE:9852 same embryo
 EurExpress:euxassay_018764 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS