EMAGE mouse gene expression spatial database

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EMAGE:9879

Bcl2l10 Bcl2-like 10 ( MGI:1330841)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008120. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008120_01	euxassay_008120_02	euxassay_008120_03	euxassay_008120_04

euxassay_008120_05	euxassay_008120_06	euxassay_008120_07	euxassay_008120_08	euxassay_008120_09

euxassay_008120_10	euxassay_008120_11	euxassay_008120_12	euxassay_008120_13	euxassay_008120_14

euxassay_008120_15	euxassay_008120_16	euxassay_008120_17	euxassay_008120_18	euxassay_008120_19

euxassay_008120_20	euxassay_008120_21	euxassay_008120_22	euxassay_008120_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9879_wholemount_strong.wlz

9879_wholemount_moderate.wlz

9879_wholemount_weak.wlz

9879_wholemount_possible.wlz

9879_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9879_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon meninges

moderate

regional

moderate expression: see section 09 10 11 15 17 18 19 weak expression: see section 12

telencephalon meninges

moderate

regional

moderate expression: see section 09 10 11 13 17 18 19 20 21 22 23 weak expression: see section 12

hindbrain meninges

moderate

regional

moderate expression: see section 09 10 11 17 18 19 20 21 22 weak expression: see section 12

midbrain meninges

moderate

regional

moderate expression: see section 09 10 11 17 18 19 20 21 weak expression: see section 12

spinal cord meninges

moderate

regional

moderate expression: see section 17 18 19 weak expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4
Entity Detected:	Bcl2l10, Bcl2-like 10 ( MGI:1330841)
Sequence:	sense strand is shown >T4 CCNNAAAATTTTTAAAGCGATCTTACCTACCTACCTGTGAACCCTCTAAGGAACAATTCTGGGTAATTGG ATAAGAACTGAGGAAGTCCTCCAGCCTAGAGACATTTCTACCTGCATGCTACATGGAGTTCTGGGTTTGT GATGGGGCCAGCATTTGAAAGGGGCCCATGTAGCTTTTCGCTGCTAAGTGAGGAAATGGTCCCCATCACT CCAGAGAACTTCAGAAAACGCTACACACACTGACTGGATGTCCAGCTGTCTGAATTTGGCTGCAGGTGCA GCAGAGCCTCCCCCATCTGTTCTGGGTTCTTGAGCTGCCAGGGTTCCAGGGCAGGGGATCCTAAAGCTCA GAGCAATGAGGAATGCAATAGGAAACTGGAAGGAATCCCTTGAAACCTAAATTCTTCTGTGCCTAGTTCT AAGGACTTCTGAATTTATAATACTCCCCTTAATACATTTTTAATAACTTCAAATTCTTAATAAATGTATG CGCTTTTTTAAAAAGGGAAAA
Notes:	The probe template was PCR amplified from IMAGE:778062 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:778062 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9877 same embryo
	EMAGE:9878 same embryo
	EMAGE:9876 same embryo
	EurExpress:euxassay_008120 same experiment
	MGI:4823449 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source