EMAGE mouse gene expression spatial database

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EMAGE:9900

Psip1 PC4 and SFRS1 interacting protein 1 ( MGI:2142116)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008131. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008131_01	euxassay_008131_02	euxassay_008131_03	euxassay_008131_04

euxassay_008131_05	euxassay_008131_06	euxassay_008131_07	euxassay_008131_08	euxassay_008131_09

euxassay_008131_10	euxassay_008131_11	euxassay_008131_12	euxassay_008131_13	euxassay_008131_14

euxassay_008131_15	euxassay_008131_16	euxassay_008131_17	euxassay_008131_18	euxassay_008131_19

euxassay_008131_20	euxassay_008131_21	euxassay_008131_22	euxassay_008131_23	euxassay_008131_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9900_wholemount_strong.wlz

9900_wholemount_moderate.wlz

9900_wholemount_weak.wlz

9900_wholemount_possible.wlz

9900_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9900_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 09 21 22 weak expression: see section 08 23

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 16

dorsal root ganglion

moderate

regional

moderate expression: see section 05 07 08 09 10 11 12 15 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20

vomeronasal organ

moderate

regional

moderate expression: see section 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T24
Entity Detected:	Psip1, PC4 and SFRS1 interacting protein 1 ( MGI:2142116)
Sequence:	sense strand is shown >T24 TTTTAGGTTGTTTCTCCTCTGGTCCCTTTTTTTTACTTTTATCTTCATCAATAACCATGTCACCGTCTGA AGGACAAGGCTGCTTTACCACTTTAGGTCTGCCTCTTGGTTTGGGAATCTTGACTTCAGTAGCTGCAGGT CGTCCTCTCTTAGGACTTGCTTTCACATTAGAAGCGGTTGCTGCAGTCACCATTCCCGCCTCTTCAGTGT CTACTTGTTTTTCAGCCTTTCTCTTTCTTCCTCGCCTGGCAGCTTTTGGAGTGGTTATGTCAACTGCTTT AGTCACATCCACGCGT
Notes:	The probe template was PCR amplified from IMAGE:943760 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:943760 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9898 same embryo
	EMAGE:9897 same embryo
	EMAGE:9899 same embryo
	EurExpress:euxassay_008131 same experiment
	MGI:4827469 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source