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EMAGE:9905

Slco4c1 solute carrier organic anion transporter family, member 4C1 ( MGI:2442784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905
"Pseudo-wholemount" of euxassay_008165. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008165_01 euxassay_008165_02 euxassay_008165_03 euxassay_008165_04
EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905
euxassay_008165_05 euxassay_008165_06 euxassay_008165_07 euxassay_008165_08 euxassay_008165_09
EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905
euxassay_008165_10 euxassay_008165_11 euxassay_008165_12 euxassay_008165_13 euxassay_008165_14
EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905 EMAGE:9905
euxassay_008165_15 euxassay_008165_16 euxassay_008165_17 euxassay_008165_18 euxassay_008165_19
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euxassay_008165_20 euxassay_008165_21 euxassay_008165_22 euxassay_008165_23 euxassay_008165_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:9905Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
9905_wholemount_strong.wlz
9905_wholemount_moderate.wlz
9905_wholemount_weak.wlz
9905_wholemount_possible.wlz
9905_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:9905_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 08 09 10 11 13 14 15 16 moderate expression: see section 17 18
renal cortex
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 16 17 18 19 21
left lung
strong strong
spottedstrong expression: see section 02 03 04 05 06 07 08 09 moderate expression: see section 01
right lung
strong strong
spottedstrong expression: see section 10 11 12 15 18 19 22 23 24 moderate expression: see section 13 14 16 17 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T50
Entity Detected:Slco4c1, solute carrier organic anion transporter family, member 4C1 ( MGI:2442784)
Sequence:sense strand is shown

>T50
TTCCCTNCCAGGTGGAGGTCTCTGCTGTGGCCTCCAGGAATCAGAATGGGGGTTCGCAGCCTCGGGAATC
TGAGGAGCCTCAGAAGTCAACTGAGCCATCCCCGCCTTCTTCGAATCCCCCAGCTTCTGATGAGCCGCCG
GGGTCACAGCTAAGCGAGCTTGAGGAGGGACCTTGCGGGTGGAGGGGCTTTCACCCCCAGTGTCTCCAGC
GCTGCAACACCCCCCAAGGCTTTTTGCTTCACTACTGTCTCTTAGCCCTAACGCAAGGTATTGTAGTAAA
CGGCCTGGTAAACATTAGCATCTCCACCATTGAGAAGCGTTATGAAATGAAAAGCTCACTGACAGGCCTG
ATATCATCGAGCTACGACATCTCCTTTTGTGTGTTATCTCTATTTGTGTCTTTCTTTGGGGAGAGAGGAC
ACAAACCTCGCTGGCTCGCCTTTGCATCCTTTATGATAGGCCTGGGAGCGCTGGTGTTTTCCTTACCACA
CTTCTTCAGTGGAAGATATGAACTGGG
Notes:The probe template was PCR amplified from IMAGE:976408 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:976408 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:9906 same embryo
 EMAGE:9903 same embryo
 EMAGE:9904 same embryo
 EurExpress:euxassay_008165 same experiment
 MGI:4828299 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS