EMAGE mouse gene expression spatial database

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EMAGE:9944

Ndufaf1 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1 ( MGI:1916952)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000196. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000196_01	euxassay_000196_02	euxassay_000196_03	euxassay_000196_04

euxassay_000196_05	euxassay_000196_06	euxassay_000196_07	euxassay_000196_08	euxassay_000196_09

euxassay_000196_10	euxassay_000196_11	euxassay_000196_12	euxassay_000196_13	euxassay_000196_14

euxassay_000196_15	euxassay_000196_16	euxassay_000196_17	euxassay_000196_18	euxassay_000196_19

euxassay_000196_20	euxassay_000196_21	euxassay_000196_22	euxassay_000196_23	euxassay_000196_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

9944_wholemount_strong.wlz

9944_wholemount_moderate.wlz

9944_wholemount_weak.wlz

9944_wholemount_possible.wlz

9944_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:9944_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T264
Entity Detected:	Ndufaf1, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1 ( MGI:1916952)
Sequence:	sense strand is shown >T264 GGCTGCATTACCTCCATTTGTGGGCAATCACTGTGTAGACTATTCTTCCAGTAGTCTTCAGAAAAAAGTG ACTTCTGTTGACAGAGCCTCCCAGCAGGGAAAGACTGAAGAGGGCTTACAAGGACATGACCACAAAGAAG TTGCTTTGGATGCACCTTCTCCTGACAGGACACCTGAAGTTAGTTTTGATAAAGCAATTAGAGATGAAGC AATAGAACATTTTAGACGTTTGAAGGATGAAATTGTGGCTCATTTGAGAGGCCCAGACGGCCGCCCACTA CAAGAGGTCATAATGGAACAAGCCAGGGTTGTCTGGCAGTTTCGTGAAAAAGAAGATTTGGATAAGTGGA TAGTGACTTCTGATAAGATAATTGGAGGTAGAAGTGAAATATTCTTGAAAATGAGCAAGAACAACCGAAG TGCCCTGCTTTATGGAACTCTGAGCTCTGAGCCTCCTCAGGATGGGGACAGTAGACAAAGTGGATACTGT GCCATGATCTCCAGGATTCCACGGGGT
Notes:	The probe template was PCR amplified from IMAGE:2654789 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2654789 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	NMRI
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:9945 same embryo
	EMAGE:9946 same embryo
	EurExpress:euxassay_000196 same experiment
	MGI:4826642 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source