EMAGE mouse gene expression spatial database

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EMAGE:10026

Sgcb sarcoglycan, beta (dystrophin-associated glycoprotein) ( MGI:1346523)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000202. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000202_01	euxassay_000202_02	euxassay_000202_03	euxassay_000202_04

euxassay_000202_05	euxassay_000202_06	euxassay_000202_07	euxassay_000202_08	euxassay_000202_09

euxassay_000202_10	euxassay_000202_11	euxassay_000202_12	euxassay_000202_13	euxassay_000202_14

euxassay_000202_15	euxassay_000202_16	euxassay_000202_17	euxassay_000202_18	euxassay_000202_19

euxassay_000202_20	euxassay_000202_21	euxassay_000202_22	euxassay_000202_23	euxassay_000202_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10026_wholemount_strong.wlz

10026_wholemount_moderate.wlz

10026_wholemount_weak.wlz

10026_wholemount_possible.wlz

10026_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10026_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

integumental system muscle

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 11 12 13 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 11 12 13 17 18 20 21 22 23 24

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 11 17 18

pons mantle layer

strong

regional

strong expression: see section 08 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 20

trigeminal v ganglion

strong

single cell

strong expression: see section 04 05 06 07 08 20 21 22 23 24

vagus x ganglion

strong

single cell

strong expression: see section 11 20

ventral grey horn

strong

single cell

strong expression: see section 12 13 17

sympathetic ganglion

strong

single cell

strong expression: see section 12

cervico-thoracic ganglion

strong

single cell

strong expression: see section 17

thoracic ganglion

strong

single cell

strong expression: see section 13 17

peripheral nerve trunk

strong

single cell

strong expression: see section 13

dorsal root ganglion

strong

single cell

strong expression: see section 18 19

tongue muscle

strong

single cell

strong expression: see section 12 13 17 18

extraembryonic component

strong

single cell

strong expression: see section 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T273
Entity Detected:	Sgcb, sarcoglycan, beta (dystrophin-associated glycoprotein) ( MGI:1346523)
Sequence:	sense strand is shown >T273 CGGGAGAAGGCTGTGGAGCGGAGGAATGTTAACAAAGAGCACAACAGCAATTTCAAAGCTGGATATATCC CCATCGATGAGGACCGGCTCCATAAGACTGGGCTGAGGGGGCGAAAGGGCAACTTAGCCATCTGCGTGAT CGTCCTCCTGTTTATCCTGGCCGTCATCAATCTACTCATTACACTTGTCATCTGGGCGGTGATCCGCATT GGGCCAAATGGGTGTGATAGCATGGAGTTCCACGAGAGCGGTCTGCTGAGGTTCAAGCAAGTGTCTGACA TGGGCGTCATCCACCCGCTTTATAAGAGCACGGTGGGAGGACGGCGGAATGAAAACCTGGTCATCACTGG CAACAACCAGCCAATTGTCTTCCAGCAAGGGACGACCAAGCTGAGTGTTGAAAAGAACAAGACCTCCATC ACCAGCGACATCGGGATGCAGTTTTTTGACCCAAGGACACACAATATCCTGTTCAGCACGGACTATGAGA CGCA
Notes:	The probe template was PCR amplified from IMAGE:2655192 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2655192 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10025 same embryo
	EurExpress:euxassay_000202 same experiment
	MGI:4828020 same experiment
	EMAGE:31506 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source