EMAGE mouse gene expression spatial database

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EMAGE:10028

Tmem59 transmembrane protein 59 ( MGI:1929278)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008205. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008205_04	euxassay_008205_05	euxassay_008205_06	euxassay_008205_07

euxassay_008205_08	euxassay_008205_09	euxassay_008205_10	euxassay_008205_11	euxassay_008205_12

euxassay_008205_13	euxassay_008205_14	euxassay_008205_15	euxassay_008205_16	euxassay_008205_17

euxassay_008205_18	euxassay_008205_19	euxassay_008205_20	euxassay_008205_21	euxassay_008205_22

euxassay_008205_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10028_wholemount_strong.wlz

10028_wholemount_moderate.wlz

10028_wholemount_weak.wlz

10028_wholemount_possible.wlz

10028_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10028_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 04 05 06 07 08 09 19 20 21 22 23

femur

strong

regional

strong expression: see section 04 05 22 23

pituitary gland

strong

regional

strong expression: see section 13 14 15

medulla oblongata floor plate

moderate

regional

moderate expression: see section 12 13

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 16 17 18

metencephalon floor plate

moderate

regional

moderate expression: see section 12 13

spinal cord floor plate

moderate

regional

moderate expression: see section 12 13

basal columns

moderate

regional

moderate expression: see section 13 14 15 16

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 12 16 20 weak expression: see section 11 19

stomach

strong

regional

strong expression: see section 04 05 06

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 17 18 19 20 21 22 23

lower jaw alveolar sulcus

strong

regional

strong expression: see section 12 13 16 18

maxilla

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 17 18 19 20 21 22 23

upper jaw alveolar sulcus

strong

regional

strong expression: see section 12 13 16 17 18

axial skeleton

strong

regional

strong expression: see section 07 08 09 10 17 18 19

orbito-sphenoid

strong

regional

strong expression: see section 04 05 23

clavicle

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T315
Entity Detected:	Tmem59, transmembrane protein 59 ( MGI:1929278)
Sequence:	sense strand is shown >T315 CAGATGAGAAACTCACAAGCACACAGGAACTACCTTGAAGAGGAAGAAAGCGATGGCTTTTTAAGATGTC TATCTCTTAACTCTGGATGGATTTTAACCACAACCCTTGTCCTCTCGGTGATGGTGTTGCTCTGGATCTG TTGTGCAGCTGTTGCTACAGCTGTAGAACAGTATGTTCCCCCTGAGAAGCTGAGTATCTATGGTGACTTG GAATTTATGAATGAACAAAAGCTGAGCAGATACCCAGCTCCTTCTCTTGTGATTGTTAGGTCTCAGACTG AAGAACATGAGGAGGCAGGGCCCCTGCCCACCAAGGTGAACCTTGCTCACTCAGAAATCTAAGCTTTTTA AAAGAGTCGTGGACACATAAATTTCCATTCCTCATAGAGCTTTTTAAGATGGTTTCATTGGACATAGGCC TTAAGAAATCACTATAAAATGCAAATAAAGTTACCAAACTCTGTGAAGACTTTATTTGCTGTGACTTTAC CTGTATTTTTCTAGTCATTTAAGATGG
Notes:	The probe template was PCR amplified from IMAGE:3153695 using vector specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3153695 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10029 same embryo
	EMAGE:10031 same embryo
	EMAGE:10030 same embryo
	EurExpress:euxassay_008205 same experiment
	MGI:4828810 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source