EMAGE mouse gene expression spatial database

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EMAGE:10068

Gba2 glucosidase beta 2 ( MGI:2654325)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000053. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000053_01	euxassay_000053_02	euxassay_000053_03	euxassay_000053_04

euxassay_000053_05	euxassay_000053_06	euxassay_000053_07	euxassay_000053_08	euxassay_000053_09

euxassay_000053_10	euxassay_000053_11	euxassay_000053_12	euxassay_000053_13	euxassay_000053_14

euxassay_000053_15	euxassay_000053_16	euxassay_000053_17	euxassay_000053_18	euxassay_000053_19

euxassay_000053_20	euxassay_000053_21	euxassay_000053_22	euxassay_000053_23	euxassay_000053_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10068_wholemount_strong.wlz

10068_wholemount_moderate.wlz

10068_wholemount_weak.wlz

10068_wholemount_possible.wlz

10068_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10068_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon floor plate

moderate

regional

moderate expression: see section 14

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall

moderate

regional

moderate expression: see section 20

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

thalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

cerebral cortex

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

corpus striatum

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 17 18 19 20 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 18

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 11 12 15 16 17 18

medulla oblongata floor plate

moderate

regional

moderate expression: see section 13

medulla oblongata lateral wall

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 19

metencephalon lateral wall

moderate

regional

moderate expression: see section 14 15 16 19

cerebellum

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

metencephalon rest of alar plate

moderate

regional

moderate expression: see section 21

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20

pons mantle layer

moderate

regional

moderate expression: see section 13 14 15 16 18 19 20

pons marginal layer

moderate

regional

moderate expression: see section 17

midbrain floor plate

moderate

regional

moderate expression: see section 13

midbrain lateral wall

moderate

regional

moderate expression: see section 21

midbrain mantle layer

moderate

regional

moderate expression: see section 07 08 10 11 13 14 15 16 18 19 20

midbrain marginal layer

moderate

regional

moderate expression: see section 12 17

tegmentum

moderate

regional

moderate expression: see section 12 13 14 15

facial vii ganglion

moderate

homogeneous

moderate expression: see section 06 07 08 22 23

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 08 21

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 24

vagus x ganglion

moderate

homogeneous

moderate expression: see section 09 10 20 21 23

vestibulocochlear viii ganglion

moderate

homogeneous

moderate expression: see section 07 08 09 19 20 21

spinal cord mantle layer

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 10 11 12 13 17 18 19 20

neural retina

moderate

homogeneous

moderate expression: see section 01 02 03 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1530
Entity Detected:	Gba2, glucosidase beta 2 ( MGI:2654325)
Sequence:	sense strand is shown >T1530 ATTCATGATACTGCTGACTGGAAGGACCTGAACCTGAAGTTTGTATTGCAAATTTATCGGGACTATTACC TGACGGGTGATCAAGGCTTCCTGNAGGACATGTGGCCTGTGTGTCTGGCTGTGATGGAGTCCGAAATGAA GTTTGACAAGGACCAAGATGGACTCATTGAGAATGGAGGCTACGCAGACCAGACCTATGATGCATGGGTC ACCACAGGCCCCAGTGCTTACTGCGGAGGGCTGTGGCTGGCGGCAGTGGCTGTAATGGTTCAGATGGCTG TTCTGTGTGGGGCCCAAGATGTCCAGGAGAGGTTTGCTTCCATTCTCTGCCGAGGCCGAGAAGCTTATGA GAGACTGCTGTGGAACGGACGCTATTACAACTACGACAGCAGCTCCCATCCTCAGTCTCGGAGCATCATG TCTGACCAGTGTGCTGGGCAGTGGTTCCTGAGGGCCTGCGGCCTGGGAGAAGGAGACACTGAAGTATTTC CTACCCTGCATGTGGTCCGTGCTCTCCAAACCATCTTT
Notes:	The probe template was PCR amplified from IMAGE:821648 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:821648 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:10066 same embryo
	EMAGE:10069 same embryo
	EMAGE:10067 same embryo
	EurExpress:euxassay_000053 same experiment
	MGI:4825009 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source