EMAGE mouse gene expression spatial database

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EMAGE:10070

Slc2a13 solute carrier family 2 (facilitated glucose transporter), member 13 ( MGI:2146030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019713. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019713_01	euxassay_019713_02	euxassay_019713_03	euxassay_019713_04

euxassay_019713_05	euxassay_019713_06	euxassay_019713_07	euxassay_019713_08	euxassay_019713_09

euxassay_019713_10	euxassay_019713_11	euxassay_019713_12	euxassay_019713_13	euxassay_019713_14

euxassay_019713_15	euxassay_019713_16	euxassay_019713_17	euxassay_019713_18	euxassay_019713_19

euxassay_019713_20	euxassay_019713_21	euxassay_019713_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10070_wholemount_strong.wlz

10070_wholemount_moderate.wlz

10070_wholemount_weak.wlz

10070_wholemount_possible.wlz

10070_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10070_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 05 06 07 08 19 20 21 22

hypothalamus mantle layer

weak

regional

weak expression: see section 07 13 14

hypothalamus ventricular layer

weak

regional

weak expression: see section 09 10 12

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 06 07 10 13 14

telencephalon mantle layer

weak

regional

weak expression: see section 01 02 03 18 19 20 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 07

medulla oblongata basal plate ventricular layer

weak

regional

weak expression: see section 08 09 10 11 12 14 15

mandible

weak

regional

weak expression: see section 03 04 05 06 07 08 09 15 16 17 18 19 20

lower jaw molar

weak

regional

weak expression: see section 05 17

maxilla

weak

regional

weak expression: see section 04 08 09 15 19

upper jaw molar

weak

regional

weak expression: see section 05 17

clavicle

weak

regional

weak expression: see section 08 09 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T56034
Entity Detected:	Slc2a13, solute carrier family 2 (facilitated glucose transporter), member 13 ( MGI:2146030)
Sequence:	sense strand is shown >T56034 GGCTGATTCAGAAGGGACAGACTCAGAAGGCCCGCCGAATTTTGTCCCAGATGCGTGGCAATCAGACCAT TGATGAGGAGTATGACAGCATCAGGAACAGTATCGAGGAGGAGGAAAAGGAAGCCACCGCAGCTGGACCT ATAATCTGCAGAATGCTGAGTTACCCCCCAACTCGCCGAGCGTTAGTTGTGGGATGTGGCTTACAGATGT TCCAGCAACTCTCGGGCATCAACACTATCATGTACTACAGCGCAACCATCCTCCAGATGTCCGGCGTGGA AGATGACAGACTTGCAATATGGCTGGCTTCGATCACAGCATTCACCAATTTCATTTTCACACTAGTGGGA GTCTGGCTCGTCGAGAAAGTGGGTCGCAGGAAGCTTACCTTTGGTAGTTTGGCAGGTACCACAGTAGCAC TGATTATCCTTGCCCTGGGATTTTTGCTCTCGGCACAGGTCTCACCACGGGTCACTTTTAGACCAACGAC TCCTTCGGATCAAAATACCACCTGCACGGGATACAGTTACTGCAATGAGTGTATGCTGGATCCAGACTGT GGTTTCTGCTACAAGATCAACGGTTCAGCTGTCATTGATTCCTCCTGTGTTCCAGTTAACAAAGCCTCTA CAACTGAAGCAGCCTGGGGCAGGTGTGATAATGAAACCAAGTTCAAAGCTGAAGGTGCTCACTGGGCTTA CAGTTTCTGCCCTACCCCCTACTCCTGGACAGCACTCGTGGGCCTGGTGTTGTACCTTGTTTTCTTTGCA CCTGCTTTCATTATTTACTTCTCAGGAATGGGACCAATGCCCTGGACCGTGAACTCTGAAATCTATCCTC TCTGGGCAAGAAGTACAGGAAATGCGTGCTCAGCTGGAATAAACTGGATTTTCAACGTGCTGGTT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GGCTGATTCAGAAGGGACAG; Reverse Primer - name:unspecified, sequence:AACCAGCACGTTGAAAATCC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828184 same experiment
	EurExpress:euxassay_019713 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source