EMAGE mouse gene expression spatial database

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EMAGE:10079

Chst8 carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 ( MGI:1916197)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_000088. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_000088_01	euxassay_000088_02	euxassay_000088_03	euxassay_000088_04

euxassay_000088_05	euxassay_000088_06	euxassay_000088_07	euxassay_000088_08	euxassay_000088_09

euxassay_000088_10	euxassay_000088_11	euxassay_000088_12	euxassay_000088_13	euxassay_000088_14

euxassay_000088_15	euxassay_000088_16	euxassay_000088_17	euxassay_000088_18	euxassay_000088_19

euxassay_000088_20	euxassay_000088_21	euxassay_000088_22	euxassay_000088_23	euxassay_000088_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

10079_wholemount_strong.wlz

10079_wholemount_moderate.wlz

10079_wholemount_weak.wlz

10079_wholemount_possible.wlz

10079_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:10079_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 05 moderate expression: see section 04 06 22 23 weak expression: see section 02 03 07 08

diencephalon

strong

regional

strong expression: see section 11 12 moderate expression: see section 10 15 16

hypothalamus

strong

regional

strong expression: see section 11 12 moderate expression: see section 08 14 15 16

diencephalon internal capsule

moderate

regional

moderate expression: see section 08 15

diencephalon lateral wall

moderate

regional

moderate expression: see section 14

cerebral cortex

moderate

regional

moderate expression: see section 16

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 16

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 19 20

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 11 17 18 19 20 23 moderate expression: see section 22

vagus x ganglion

strong

homogeneous

strong expression: see section 06 07 08 18 19 20

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 04 06 07 08 18 19 20

facial vii nerve

strong

regional

strong expression: see section 05

trigeminal v nerve

strong

homogeneous

strong expression: see section 06

trigeminal v nerve mandibular division

strong

regional

strong expression: see section 06 07 08

trigeminal v nerve maxillary division

strong

homogeneous

strong expression: see section 06 11 moderate expression: see section 12

trigeminal v nerve ophthalmic division

strong

homogeneous

strong expression: see section 06 11

dorsal root ganglion

strong

homogeneous

strong expression: see section 10 11 15 17 18 19 20 moderate expression: see section 12 16

heart

strong

single cell

strong expression: see section 16 18 19 moderate expression: see section 11 12 weak expression: see section 14

heart ventricle

moderate

single cell

moderate expression: see section 11 12

bladder

strong

regional

strong expression: see section 15

pectoral girdle and thoracic body wall skeleton

strong

regional

strong expression: see section 05 24 moderate expression: see section 01 04 06 weak expression: see section 03

pelvic girdle skeleton

strong

regional

strong expression: see section 02 24 moderate expression: see section 01 04 06 weak expression: see section 03 07 08

acetabular region

strong

regional

strong expression: see section 05 weak expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1618
Entity Detected:	Chst8, carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 8 ( MGI:1916197)
Sequence:	sense strand is shown >T1618 TGGCCTCGAGGCAGATTCGGACGAGGCTTCTTCCTGCGTCTCATCCATGCGCCCGGGAACCTGACTTTCC CGAGGTTCAAGGACAGGCACTCCGAGGAGGCGCGGACCACATCGAGAATCACCCATCAGTACTTCGCTCA GCTCTCCTCGCTGCAGCGACAGCGAACCTACGACTTCTACTACATGGATTACCTGATGTTCAACTACTCC AAACCTTTCTCGGACCTGTACTGAGGGCGGGGCCTGCTGGTCAGGGGCGGGGTCTGCCGGTCATGCCCAC TCACCTGCGCATAGGCGGCCTCCGGGGACTGAGCTCTGAGGATGTGAGGCCTTGTGGCTGTGGCCCTAGG GTGGGCCACAGAGGCCCAGACAATGGACCTTGACCCTTGTCCCACACCCATTTCCTCATTGGGTTGGCTG AGTTTGAGACGGAGCACGACTCGGATGGATGCTTTAAGAACTCAGCTGAGCTATGCTGAGCTATGCTGTC CAGGGAAGCCTGAGACCCAGAAGAGGGCCCCAGCGTCGAGGGATGTCCTACATCCCCT
Notes:	The probe template was PCR amplified from IMAGE:426351 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:426351 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4823870 same experiment
	EurExpress:euxassay_000088 same experiment
	EMAGE:31517 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source